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Expression pattern and activity of six glutelin gene promoters in transgenic rice*

The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, Gl...

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Autores principales: Qu, Le Qing, Xing, Yan Ping, Liu, Wen Xian, Xu, Xiu Ping, Song, Yan Ru
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423653/
https://www.ncbi.nlm.nih.gov/pubmed/18467323
http://dx.doi.org/10.1093/jxb/ern110
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author Qu, Le Qing
Xing, Yan Ping
Liu, Wen Xian
Xu, Xiu Ping
Song, Yan Ru
author_facet Qu, Le Qing
Xing, Yan Ping
Liu, Wen Xian
Xu, Xiu Ping
Song, Yan Ru
author_sort Qu, Le Qing
collection PubMed
description The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, GluB-5, and GluC) were isolated from rice (Oryza sativa L.) genomic DNA by PCR. Their spatial and temporal expression patterns and expression potential in stable transgenic rice plants were examined with β-glucuronidase (GUS) used as a reporter gene. All the promoters showed the expected spatial expression within the endosperm. The GluA-1, GluA-2, and GluA-3 promoters directed GUS expression mainly in the outer portion (peripheral region) of the endosperm. The GluB-5 and GluC promoters directed GUS expression in the whole endosperm, with the latter expressed almost evenly throughout the whole endosperm, a feature different from that of other rice glutelin gene promoters. The GluB-3 promoter directed GUS expression solely in aleurone and subaleurone layers. Promoter activities examined during seed maturation showed that the GluC promoter had much higher activity than the other promoters. These promoters are ideal candidates for achieving gene expression for multiple purposes in monocot endosperm but avoid promoter homology-based gene silencing. The GluC promoter did not contain the endosperm specificity-determining motifs GCN4, AACA, and the prolamin-box, which suggests the existence of additional regulatory mechanism in determining endosperm specificity.
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spelling pubmed-24236532009-02-25 Expression pattern and activity of six glutelin gene promoters in transgenic rice* Qu, Le Qing Xing, Yan Ping Liu, Wen Xian Xu, Xiu Ping Song, Yan Ru J Exp Bot Research Papers The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, GluB-5, and GluC) were isolated from rice (Oryza sativa L.) genomic DNA by PCR. Their spatial and temporal expression patterns and expression potential in stable transgenic rice plants were examined with β-glucuronidase (GUS) used as a reporter gene. All the promoters showed the expected spatial expression within the endosperm. The GluA-1, GluA-2, and GluA-3 promoters directed GUS expression mainly in the outer portion (peripheral region) of the endosperm. The GluB-5 and GluC promoters directed GUS expression in the whole endosperm, with the latter expressed almost evenly throughout the whole endosperm, a feature different from that of other rice glutelin gene promoters. The GluB-3 promoter directed GUS expression solely in aleurone and subaleurone layers. Promoter activities examined during seed maturation showed that the GluC promoter had much higher activity than the other promoters. These promoters are ideal candidates for achieving gene expression for multiple purposes in monocot endosperm but avoid promoter homology-based gene silencing. The GluC promoter did not contain the endosperm specificity-determining motifs GCN4, AACA, and the prolamin-box, which suggests the existence of additional regulatory mechanism in determining endosperm specificity. Oxford University Press 2008-06 2008-05-07 /pmc/articles/PMC2423653/ /pubmed/18467323 http://dx.doi.org/10.1093/jxb/ern110 Text en © 2008 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Qu, Le Qing
Xing, Yan Ping
Liu, Wen Xian
Xu, Xiu Ping
Song, Yan Ru
Expression pattern and activity of six glutelin gene promoters in transgenic rice*
title Expression pattern and activity of six glutelin gene promoters in transgenic rice*
title_full Expression pattern and activity of six glutelin gene promoters in transgenic rice*
title_fullStr Expression pattern and activity of six glutelin gene promoters in transgenic rice*
title_full_unstemmed Expression pattern and activity of six glutelin gene promoters in transgenic rice*
title_short Expression pattern and activity of six glutelin gene promoters in transgenic rice*
title_sort expression pattern and activity of six glutelin gene promoters in transgenic rice*
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2423653/
https://www.ncbi.nlm.nih.gov/pubmed/18467323
http://dx.doi.org/10.1093/jxb/ern110
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