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UniPrime: a workflow-based platform for improved universal primer design
UniPrime is an open-source software (http://uniprime.batlab.eu), which automatically designs large sets of universal primers by simply inputting a gene ID reference. UniPrime automatically retrieves and aligns homologous sequences from GenBank, identifies regions of conservation within the alignment...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2425486/ https://www.ncbi.nlm.nih.gov/pubmed/18424794 http://dx.doi.org/10.1093/nar/gkn191 |
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author | Bekaert, Michaël Teeling, Emma C. |
author_facet | Bekaert, Michaël Teeling, Emma C. |
author_sort | Bekaert, Michaël |
collection | PubMed |
description | UniPrime is an open-source software (http://uniprime.batlab.eu), which automatically designs large sets of universal primers by simply inputting a gene ID reference. UniPrime automatically retrieves and aligns homologous sequences from GenBank, identifies regions of conservation within the alignment and generates suitable primers that can amplify variable genomic regions. UniPrime differs from previous automatic primer design programs in that all steps of primer design are automated, saved and are phylogenetically limited. We have experimentally verified the efficiency and success of this program by amplifying and sequencing four diverse genes (AOF2, EFEMP1, LRP6 and OAZ1) across multiple Orders of mammals. UniPrime is an experimentally validated, fully automated program that generates successful cross-species primers that take into account the biological aspects of the PCR. |
format | Text |
id | pubmed-2425486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-24254862008-06-12 UniPrime: a workflow-based platform for improved universal primer design Bekaert, Michaël Teeling, Emma C. Nucleic Acids Res Methods Online UniPrime is an open-source software (http://uniprime.batlab.eu), which automatically designs large sets of universal primers by simply inputting a gene ID reference. UniPrime automatically retrieves and aligns homologous sequences from GenBank, identifies regions of conservation within the alignment and generates suitable primers that can amplify variable genomic regions. UniPrime differs from previous automatic primer design programs in that all steps of primer design are automated, saved and are phylogenetically limited. We have experimentally verified the efficiency and success of this program by amplifying and sequencing four diverse genes (AOF2, EFEMP1, LRP6 and OAZ1) across multiple Orders of mammals. UniPrime is an experimentally validated, fully automated program that generates successful cross-species primers that take into account the biological aspects of the PCR. Oxford University Press 2008-06 2008-04-19 /pmc/articles/PMC2425486/ /pubmed/18424794 http://dx.doi.org/10.1093/nar/gkn191 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methods Online Bekaert, Michaël Teeling, Emma C. UniPrime: a workflow-based platform for improved universal primer design |
title | UniPrime: a workflow-based platform for improved universal primer design |
title_full | UniPrime: a workflow-based platform for improved universal primer design |
title_fullStr | UniPrime: a workflow-based platform for improved universal primer design |
title_full_unstemmed | UniPrime: a workflow-based platform for improved universal primer design |
title_short | UniPrime: a workflow-based platform for improved universal primer design |
title_sort | uniprime: a workflow-based platform for improved universal primer design |
topic | Methods Online |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2425486/ https://www.ncbi.nlm.nih.gov/pubmed/18424794 http://dx.doi.org/10.1093/nar/gkn191 |
work_keys_str_mv | AT bekaertmichael uniprimeaworkflowbasedplatformforimproveduniversalprimerdesign AT teelingemmac uniprimeaworkflowbasedplatformforimproveduniversalprimerdesign |