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Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons
Genotyping by high-resolution melting analysis of small amplicons is homogeneous and simple. However, this approach can be limited by physical and chemical components of the system that contribute to intersample melting variation. It is challenging for this method to distinguish homozygous G::C from...
Autores principales: | Gundry, Cameron N., Dobrowolski, Steven F., Martin, Y. Ranae, Robbins, Thomas C., Nay, Lyle M., Boyd, Nathan, Coyne, Thomas, Wall, Mikeal D., Wittwer, Carl T., Teng, David H.-F. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2425497/ https://www.ncbi.nlm.nih.gov/pubmed/18448472 http://dx.doi.org/10.1093/nar/gkn204 |
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