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Geometric control of myogenic cell fate

This work combines expertise in stem cell biology and bioengineering to define the system for geometric control of proliferation and differentiation of myogenic progenitor cells. We have created an artificial niche of myogenic progenitor cells, namely, modified extracellular matrix (ECM) substrates...

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Detalles Bibliográficos
Autores principales: de Juan-Pardo, Elena M, Hoang, Mike Bao-Trong, Conboy, Irina M
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426793/
https://www.ncbi.nlm.nih.gov/pubmed/17722537
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author de Juan-Pardo, Elena M
Hoang, Mike Bao-Trong
Conboy, Irina M
author_facet de Juan-Pardo, Elena M
Hoang, Mike Bao-Trong
Conboy, Irina M
author_sort de Juan-Pardo, Elena M
collection PubMed
description This work combines expertise in stem cell biology and bioengineering to define the system for geometric control of proliferation and differentiation of myogenic progenitor cells. We have created an artificial niche of myogenic progenitor cells, namely, modified extracellular matrix (ECM) substrates with spatially embedded growth or differentiation factors (GF, DF) that predictably direct muscle cell fate in a geometric pattern. Embedded GF and DF signal progenitor cells from specifically defined areas on the ECM successfully competed against culture media for myogenic cell fate determination at a clearly defined boundary. Differentiation of myoblasts into myotubes is induced in growth-promoting medium, myotube formation is delayed in differentiation-promoting medium, and myogenic cells, at different stages of proliferation and differentiation, can be induced to coexist adjacently in identical culture media. This method can be used to identify molecular interactions between cells in different stages of myogenic differentiation, which are likely to be important determinants of tissue repair. The designed ECM niches can be further developed into a vehicle for transplantation of myogenic progenitor cells maintaining their regenerative potential. Additionally, this work may also serve as a general model to engineer synthetic cellular niches to harness the regenerative potential of organ stem cells.
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spelling pubmed-24267932008-06-20 Geometric control of myogenic cell fate de Juan-Pardo, Elena M Hoang, Mike Bao-Trong Conboy, Irina M Int J Nanomedicine New Technologies This work combines expertise in stem cell biology and bioengineering to define the system for geometric control of proliferation and differentiation of myogenic progenitor cells. We have created an artificial niche of myogenic progenitor cells, namely, modified extracellular matrix (ECM) substrates with spatially embedded growth or differentiation factors (GF, DF) that predictably direct muscle cell fate in a geometric pattern. Embedded GF and DF signal progenitor cells from specifically defined areas on the ECM successfully competed against culture media for myogenic cell fate determination at a clearly defined boundary. Differentiation of myoblasts into myotubes is induced in growth-promoting medium, myotube formation is delayed in differentiation-promoting medium, and myogenic cells, at different stages of proliferation and differentiation, can be induced to coexist adjacently in identical culture media. This method can be used to identify molecular interactions between cells in different stages of myogenic differentiation, which are likely to be important determinants of tissue repair. The designed ECM niches can be further developed into a vehicle for transplantation of myogenic progenitor cells maintaining their regenerative potential. Additionally, this work may also serve as a general model to engineer synthetic cellular niches to harness the regenerative potential of organ stem cells. Dove Medical Press 2006-06 /pmc/articles/PMC2426793/ /pubmed/17722537 Text en © 2006 Dove Medical Press Limited. All rights reserved
spellingShingle New Technologies
de Juan-Pardo, Elena M
Hoang, Mike Bao-Trong
Conboy, Irina M
Geometric control of myogenic cell fate
title Geometric control of myogenic cell fate
title_full Geometric control of myogenic cell fate
title_fullStr Geometric control of myogenic cell fate
title_full_unstemmed Geometric control of myogenic cell fate
title_short Geometric control of myogenic cell fate
title_sort geometric control of myogenic cell fate
topic New Technologies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426793/
https://www.ncbi.nlm.nih.gov/pubmed/17722537
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