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Efficient gene transfection using chitosan–alginate core-shell nanoparticles

Reverse microemulsion was used as a template to fabricate chitosan–alginate core-shell nanoparticles encapsulated with enhanced green fluorescent protein (EGFP)-encoded plasmids. The average size of DNA-entrapped nanoparticles measured by dynamic light scattering was increased proportionally, with t...

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Detalles Bibliográficos
Autores principales: You, Jin-Oh, Liu, Yu-Chuan, Peng, Ching-An
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426794/
https://www.ncbi.nlm.nih.gov/pubmed/17722533
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author You, Jin-Oh
Liu, Yu-Chuan
Peng, Ching-An
author_facet You, Jin-Oh
Liu, Yu-Chuan
Peng, Ching-An
author_sort You, Jin-Oh
collection PubMed
description Reverse microemulsion was used as a template to fabricate chitosan–alginate core-shell nanoparticles encapsulated with enhanced green fluorescent protein (EGFP)-encoded plasmids. The average size of DNA-entrapped nanoparticles measured by dynamic light scattering was increased proportionally, with the N/P ratios ranging from 5 to 20. These alginate-coated chitosan nanoparticles endocytosed by NIH 3T3 cells trigged swelling of transport vesicles which render gene escape before entering digestive endolysosomal compartment and concomitantly promote gene transfection rate. Results showed that DNA-encapsulated chitosan–alginate nanoparticles with average size of 64 nm (N/P ratio of 5) could achieve the level of gene expression comparable with the one obtained by using polyethyleneimine–DNA complexes.
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spelling pubmed-24267942008-06-20 Efficient gene transfection using chitosan–alginate core-shell nanoparticles You, Jin-Oh Liu, Yu-Chuan Peng, Ching-An Int J Nanomedicine Original Research Reverse microemulsion was used as a template to fabricate chitosan–alginate core-shell nanoparticles encapsulated with enhanced green fluorescent protein (EGFP)-encoded plasmids. The average size of DNA-entrapped nanoparticles measured by dynamic light scattering was increased proportionally, with the N/P ratios ranging from 5 to 20. These alginate-coated chitosan nanoparticles endocytosed by NIH 3T3 cells trigged swelling of transport vesicles which render gene escape before entering digestive endolysosomal compartment and concomitantly promote gene transfection rate. Results showed that DNA-encapsulated chitosan–alginate nanoparticles with average size of 64 nm (N/P ratio of 5) could achieve the level of gene expression comparable with the one obtained by using polyethyleneimine–DNA complexes. Dove Medical Press 2006-06 /pmc/articles/PMC2426794/ /pubmed/17722533 Text en © 2006 Dove Medical Press Limited. All rights reserved
spellingShingle Original Research
You, Jin-Oh
Liu, Yu-Chuan
Peng, Ching-An
Efficient gene transfection using chitosan–alginate core-shell nanoparticles
title Efficient gene transfection using chitosan–alginate core-shell nanoparticles
title_full Efficient gene transfection using chitosan–alginate core-shell nanoparticles
title_fullStr Efficient gene transfection using chitosan–alginate core-shell nanoparticles
title_full_unstemmed Efficient gene transfection using chitosan–alginate core-shell nanoparticles
title_short Efficient gene transfection using chitosan–alginate core-shell nanoparticles
title_sort efficient gene transfection using chitosan–alginate core-shell nanoparticles
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426794/
https://www.ncbi.nlm.nih.gov/pubmed/17722533
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