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Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape

Plectin is a versatile intermediate filament (IF)–bound cytolinker protein with a variety of differentially spliced isoforms accounting for its multiple functions. One particular isoform, plectin 1b (P1b), remains associated with mitochondria after biochemical fractionation of fibroblasts and cells...

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Detalles Bibliográficos
Autores principales: Winter, Lilli, Abrahamsberg, Christina, Wiche, Gerhard
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426950/
https://www.ncbi.nlm.nih.gov/pubmed/18541706
http://dx.doi.org/10.1083/jcb.200710151
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author Winter, Lilli
Abrahamsberg, Christina
Wiche, Gerhard
author_facet Winter, Lilli
Abrahamsberg, Christina
Wiche, Gerhard
author_sort Winter, Lilli
collection PubMed
description Plectin is a versatile intermediate filament (IF)–bound cytolinker protein with a variety of differentially spliced isoforms accounting for its multiple functions. One particular isoform, plectin 1b (P1b), remains associated with mitochondria after biochemical fractionation of fibroblasts and cells expressing exogenous P1b. Here, we determined that P1b is inserted into the outer mitochondrial membrane with the exon 1b–encoded N-terminal sequence serving as a mitochondrial targeting and anchoring signal. To study P1b-related mitochondrial functions, we generated mice that selectively lack this isoform but express all others. In primary fibroblasts and myoblasts derived from these mice, we observe a substantial elongation of mitochondrial networks, whereas other mitochondrial properties remain largely unaffected. Normal morphology of mitochondria could be restored by isoform-specific overexpression of P1b in P1b-deficient as well as plectin-null cells. We propose a model where P1b both forms a mitochondrial signaling platform and affects organelle shape and network formation by tethering mitochondria to IFs.
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spelling pubmed-24269502008-12-16 Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape Winter, Lilli Abrahamsberg, Christina Wiche, Gerhard J Cell Biol Research Articles Plectin is a versatile intermediate filament (IF)–bound cytolinker protein with a variety of differentially spliced isoforms accounting for its multiple functions. One particular isoform, plectin 1b (P1b), remains associated with mitochondria after biochemical fractionation of fibroblasts and cells expressing exogenous P1b. Here, we determined that P1b is inserted into the outer mitochondrial membrane with the exon 1b–encoded N-terminal sequence serving as a mitochondrial targeting and anchoring signal. To study P1b-related mitochondrial functions, we generated mice that selectively lack this isoform but express all others. In primary fibroblasts and myoblasts derived from these mice, we observe a substantial elongation of mitochondrial networks, whereas other mitochondrial properties remain largely unaffected. Normal morphology of mitochondria could be restored by isoform-specific overexpression of P1b in P1b-deficient as well as plectin-null cells. We propose a model where P1b both forms a mitochondrial signaling platform and affects organelle shape and network formation by tethering mitochondria to IFs. The Rockefeller University Press 2008-06-16 /pmc/articles/PMC2426950/ /pubmed/18541706 http://dx.doi.org/10.1083/jcb.200710151 Text en © 2008 Winter et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Winter, Lilli
Abrahamsberg, Christina
Wiche, Gerhard
Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
title Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
title_full Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
title_fullStr Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
title_full_unstemmed Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
title_short Plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
title_sort plectin isoform 1b mediates mitochondrion–intermediate filament network linkage and controls organelle shape
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2426950/
https://www.ncbi.nlm.nih.gov/pubmed/18541706
http://dx.doi.org/10.1083/jcb.200710151
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