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A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter

The construction of expression vectors derived from the human adenovirus type 5 (Ad5), usually based on homologous recombination, is time consuming as a shuttle plasmid has to be selected before recombination with the viral genome. Here, we describe a method allowing direct cloning of a transgene in...

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Detalles Bibliográficos
Autores principales: Mailly, Laurent, Boulade-Ladame, Charlotte, Orfanoudakis, Georges, Deryckere, François
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2427025/
https://www.ncbi.nlm.nih.gov/pubmed/18538014
http://dx.doi.org/10.1186/1743-422X-5-73
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author Mailly, Laurent
Boulade-Ladame, Charlotte
Orfanoudakis, Georges
Deryckere, François
author_facet Mailly, Laurent
Boulade-Ladame, Charlotte
Orfanoudakis, Georges
Deryckere, François
author_sort Mailly, Laurent
collection PubMed
description The construction of expression vectors derived from the human adenovirus type 5 (Ad5), usually based on homologous recombination, is time consuming as a shuttle plasmid has to be selected before recombination with the viral genome. Here, we describe a method allowing direct cloning of a transgene in the E3 region of the Ad5 genome already containing the immediate early CMV promoter upstream of three unique restriction sites. This allowed the construction of recombinant adenoviral genomes in just one step, reducing considerably the time of selection and, of course, production of the corresponding vectors. Using this vector, we produced recombinant adenoviruses, each giving high-level expression of the transgene in the transduced cells.
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spelling pubmed-24270252008-06-13 A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter Mailly, Laurent Boulade-Ladame, Charlotte Orfanoudakis, Georges Deryckere, François Virol J Short Report The construction of expression vectors derived from the human adenovirus type 5 (Ad5), usually based on homologous recombination, is time consuming as a shuttle plasmid has to be selected before recombination with the viral genome. Here, we describe a method allowing direct cloning of a transgene in the E3 region of the Ad5 genome already containing the immediate early CMV promoter upstream of three unique restriction sites. This allowed the construction of recombinant adenoviral genomes in just one step, reducing considerably the time of selection and, of course, production of the corresponding vectors. Using this vector, we produced recombinant adenoviruses, each giving high-level expression of the transgene in the transduced cells. BioMed Central 2008-06-06 /pmc/articles/PMC2427025/ /pubmed/18538014 http://dx.doi.org/10.1186/1743-422X-5-73 Text en Copyright © 2008 Mailly et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Mailly, Laurent
Boulade-Ladame, Charlotte
Orfanoudakis, Georges
Deryckere, François
A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
title A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
title_full A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
title_fullStr A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
title_full_unstemmed A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
title_short A novel adenovirus vector for easy cloning in the E3 region downstream of the CMV promoter
title_sort novel adenovirus vector for easy cloning in the e3 region downstream of the cmv promoter
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2427025/
https://www.ncbi.nlm.nih.gov/pubmed/18538014
http://dx.doi.org/10.1186/1743-422X-5-73
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