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Protecting role of cosolvents in protein denaturation by SDS: a structural study

BACKGROUND: Recently, we reported a unique approach to preserve the activity of some proteins in the presence of the denaturing agent, Sodium Dodecyl Sulfate (SDS). This was made possible by addition of the amphipathic solvent 2,4-Methyl-2-PentaneDiol (MPD), used as protecting but also as refolding...

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Detalles Bibliográficos
Autores principales: Michaux, Catherine, Pouyez, Jenny, Wouters, Johan, Privé, Gilbert G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2429906/
https://www.ncbi.nlm.nih.gov/pubmed/18522744
http://dx.doi.org/10.1186/1472-6807-8-29

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