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Protecting role of cosolvents in protein denaturation by SDS: a structural study
BACKGROUND: Recently, we reported a unique approach to preserve the activity of some proteins in the presence of the denaturing agent, Sodium Dodecyl Sulfate (SDS). This was made possible by addition of the amphipathic solvent 2,4-Methyl-2-PentaneDiol (MPD), used as protecting but also as refolding...
Autores principales: | Michaux, Catherine, Pouyez, Jenny, Wouters, Johan, Privé, Gilbert G |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2429906/ https://www.ncbi.nlm.nih.gov/pubmed/18522744 http://dx.doi.org/10.1186/1472-6807-8-29 |
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