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Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite

Proliferation of the pathogenic Plasmodium asexual blood stages in host erythrocytes requires an exquisite capacity to protect the malaria parasite against oxidative stress. This function is achieved by a complex antioxidant defence system composed of redox-active proteins and low MW antioxidants. H...

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Autores principales: Buchholz, Kathrin, Rahlfs, Stefan, Schirmer, R. Heiner, Becker, Katja, Matuschewski, Kai
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2429964/
https://www.ncbi.nlm.nih.gov/pubmed/18575607
http://dx.doi.org/10.1371/journal.pone.0002474
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author Buchholz, Kathrin
Rahlfs, Stefan
Schirmer, R. Heiner
Becker, Katja
Matuschewski, Kai
author_facet Buchholz, Kathrin
Rahlfs, Stefan
Schirmer, R. Heiner
Becker, Katja
Matuschewski, Kai
author_sort Buchholz, Kathrin
collection PubMed
description Proliferation of the pathogenic Plasmodium asexual blood stages in host erythrocytes requires an exquisite capacity to protect the malaria parasite against oxidative stress. This function is achieved by a complex antioxidant defence system composed of redox-active proteins and low MW antioxidants. Here, we disrupted the P. berghei plasmoredoxin gene that encodes a parasite-specific 22 kDa member of the thioredoxin superfamily. The successful generation of plasmoredoxin knockout mutants in the rodent model malaria parasite and phenotypic analysis during life cycle progression revealed a non-vital role in vivo. Our findings suggest that plasmoredoxin fulfils a specialized and dispensable role for Plasmodium and highlights the need for target validation to inform drug development strategies.
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spelling pubmed-24299642008-06-25 Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite Buchholz, Kathrin Rahlfs, Stefan Schirmer, R. Heiner Becker, Katja Matuschewski, Kai PLoS One Research Article Proliferation of the pathogenic Plasmodium asexual blood stages in host erythrocytes requires an exquisite capacity to protect the malaria parasite against oxidative stress. This function is achieved by a complex antioxidant defence system composed of redox-active proteins and low MW antioxidants. Here, we disrupted the P. berghei plasmoredoxin gene that encodes a parasite-specific 22 kDa member of the thioredoxin superfamily. The successful generation of plasmoredoxin knockout mutants in the rodent model malaria parasite and phenotypic analysis during life cycle progression revealed a non-vital role in vivo. Our findings suggest that plasmoredoxin fulfils a specialized and dispensable role for Plasmodium and highlights the need for target validation to inform drug development strategies. Public Library of Science 2008-06-25 /pmc/articles/PMC2429964/ /pubmed/18575607 http://dx.doi.org/10.1371/journal.pone.0002474 Text en Buchholz et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Buchholz, Kathrin
Rahlfs, Stefan
Schirmer, R. Heiner
Becker, Katja
Matuschewski, Kai
Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite
title Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite
title_full Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite
title_fullStr Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite
title_full_unstemmed Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite
title_short Depletion of Plasmodium berghei Plasmoredoxin Reveals a Non-Essential Role for Life Cycle Progression of the Malaria Parasite
title_sort depletion of plasmodium berghei plasmoredoxin reveals a non-essential role for life cycle progression of the malaria parasite
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2429964/
https://www.ncbi.nlm.nih.gov/pubmed/18575607
http://dx.doi.org/10.1371/journal.pone.0002474
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