Cargando…

The Aedes aegypti Toll Pathway Controls Dengue Virus Infection

Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila me...

Descripción completa

Detalles Bibliográficos
Autores principales: Xi, Zhiyong, Ramirez, Jose L., Dimopoulos, George
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435278/
https://www.ncbi.nlm.nih.gov/pubmed/18604274
http://dx.doi.org/10.1371/journal.ppat.1000098
_version_ 1782156477920706560
author Xi, Zhiyong
Ramirez, Jose L.
Dimopoulos, George
author_facet Xi, Zhiyong
Ramirez, Jose L.
Dimopoulos, George
author_sort Xi, Zhiyong
collection PubMed
description Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila melanogaster, which appears to utilize different immune pathways against different types of viruses, in addition to an RNA interference–based defense system. We have used the recently released whole-genome sequence of the Ae. aegypti mosquito, in combination with high-throughput gene expression and RNA interference (RNAi)-based reverse genetic analyses, to characterize its response to dengue virus infection in different body compartments. We have further addressed the impact of the mosquito's endogenous microbial flora on virus infection. Our findings indicate a significant role for the Toll pathway in regulating resistance to dengue virus, as indicated by an infection-responsive regulation and functional assessment of several Toll pathway–associated genes. We have also shown that the mosquito's natural microbiota play a role in modulating the dengue virus infection, possibly through basal-level stimulation of the Toll immune pathway.
format Text
id pubmed-2435278
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-24352782008-07-04 The Aedes aegypti Toll Pathway Controls Dengue Virus Infection Xi, Zhiyong Ramirez, Jose L. Dimopoulos, George PLoS Pathog Research Article Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila melanogaster, which appears to utilize different immune pathways against different types of viruses, in addition to an RNA interference–based defense system. We have used the recently released whole-genome sequence of the Ae. aegypti mosquito, in combination with high-throughput gene expression and RNA interference (RNAi)-based reverse genetic analyses, to characterize its response to dengue virus infection in different body compartments. We have further addressed the impact of the mosquito's endogenous microbial flora on virus infection. Our findings indicate a significant role for the Toll pathway in regulating resistance to dengue virus, as indicated by an infection-responsive regulation and functional assessment of several Toll pathway–associated genes. We have also shown that the mosquito's natural microbiota play a role in modulating the dengue virus infection, possibly through basal-level stimulation of the Toll immune pathway. Public Library of Science 2008-07-04 /pmc/articles/PMC2435278/ /pubmed/18604274 http://dx.doi.org/10.1371/journal.ppat.1000098 Text en Xi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xi, Zhiyong
Ramirez, Jose L.
Dimopoulos, George
The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
title The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
title_full The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
title_fullStr The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
title_full_unstemmed The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
title_short The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
title_sort aedes aegypti toll pathway controls dengue virus infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435278/
https://www.ncbi.nlm.nih.gov/pubmed/18604274
http://dx.doi.org/10.1371/journal.ppat.1000098
work_keys_str_mv AT xizhiyong theaedesaegyptitollpathwaycontrolsdenguevirusinfection
AT ramirezjosel theaedesaegyptitollpathwaycontrolsdenguevirusinfection
AT dimopoulosgeorge theaedesaegyptitollpathwaycontrolsdenguevirusinfection
AT xizhiyong aedesaegyptitollpathwaycontrolsdenguevirusinfection
AT ramirezjosel aedesaegyptitollpathwaycontrolsdenguevirusinfection
AT dimopoulosgeorge aedesaegyptitollpathwaycontrolsdenguevirusinfection