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The Aedes aegypti Toll Pathway Controls Dengue Virus Infection
Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila me...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435278/ https://www.ncbi.nlm.nih.gov/pubmed/18604274 http://dx.doi.org/10.1371/journal.ppat.1000098 |
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author | Xi, Zhiyong Ramirez, Jose L. Dimopoulos, George |
author_facet | Xi, Zhiyong Ramirez, Jose L. Dimopoulos, George |
author_sort | Xi, Zhiyong |
collection | PubMed |
description | Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila melanogaster, which appears to utilize different immune pathways against different types of viruses, in addition to an RNA interference–based defense system. We have used the recently released whole-genome sequence of the Ae. aegypti mosquito, in combination with high-throughput gene expression and RNA interference (RNAi)-based reverse genetic analyses, to characterize its response to dengue virus infection in different body compartments. We have further addressed the impact of the mosquito's endogenous microbial flora on virus infection. Our findings indicate a significant role for the Toll pathway in regulating resistance to dengue virus, as indicated by an infection-responsive regulation and functional assessment of several Toll pathway–associated genes. We have also shown that the mosquito's natural microbiota play a role in modulating the dengue virus infection, possibly through basal-level stimulation of the Toll immune pathway. |
format | Text |
id | pubmed-2435278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-24352782008-07-04 The Aedes aegypti Toll Pathway Controls Dengue Virus Infection Xi, Zhiyong Ramirez, Jose L. Dimopoulos, George PLoS Pathog Research Article Aedes aegypti, the mosquito vector of dengue viruses, utilizes its innate immune system to ward off a variety of pathogens, some of which can cause disease in humans. To date, the features of insects' innate immune defenses against viruses have mainly been studied in the fruit fly Drosophila melanogaster, which appears to utilize different immune pathways against different types of viruses, in addition to an RNA interference–based defense system. We have used the recently released whole-genome sequence of the Ae. aegypti mosquito, in combination with high-throughput gene expression and RNA interference (RNAi)-based reverse genetic analyses, to characterize its response to dengue virus infection in different body compartments. We have further addressed the impact of the mosquito's endogenous microbial flora on virus infection. Our findings indicate a significant role for the Toll pathway in regulating resistance to dengue virus, as indicated by an infection-responsive regulation and functional assessment of several Toll pathway–associated genes. We have also shown that the mosquito's natural microbiota play a role in modulating the dengue virus infection, possibly through basal-level stimulation of the Toll immune pathway. Public Library of Science 2008-07-04 /pmc/articles/PMC2435278/ /pubmed/18604274 http://dx.doi.org/10.1371/journal.ppat.1000098 Text en Xi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Xi, Zhiyong Ramirez, Jose L. Dimopoulos, George The Aedes aegypti Toll Pathway Controls Dengue Virus Infection |
title | The Aedes aegypti Toll Pathway Controls Dengue Virus Infection |
title_full | The Aedes aegypti Toll Pathway Controls Dengue Virus Infection |
title_fullStr | The Aedes aegypti Toll Pathway Controls Dengue Virus Infection |
title_full_unstemmed | The Aedes aegypti Toll Pathway Controls Dengue Virus Infection |
title_short | The Aedes aegypti Toll Pathway Controls Dengue Virus Infection |
title_sort | aedes aegypti toll pathway controls dengue virus infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435278/ https://www.ncbi.nlm.nih.gov/pubmed/18604274 http://dx.doi.org/10.1371/journal.ppat.1000098 |
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