Cargando…

Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium

BACKGROUND: Cigarette smoking is a leading cause of preventable death and a significant cause of lung cancer and chronic obstructive pulmonary disease. Prior studies have demonstrated that smoking creates a field of molecular injury throughout the airway epithelium exposed to cigarette smoke. We hav...

Descripción completa

Detalles Bibliográficos
Autores principales: Sridhar, Sriram, Schembri, Frank, Zeskind, Julie, Shah, Vishal, Gustafson, Adam M, Steiling, Katrina, Liu, Gang, Dumas, Yves-Martine, Zhang, Xiaohui, Brody, Jerome S, Lenburg, Marc E, Spira, Avrum
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435556/
https://www.ncbi.nlm.nih.gov/pubmed/18513428
http://dx.doi.org/10.1186/1471-2164-9-259
_version_ 1782156494871986176
author Sridhar, Sriram
Schembri, Frank
Zeskind, Julie
Shah, Vishal
Gustafson, Adam M
Steiling, Katrina
Liu, Gang
Dumas, Yves-Martine
Zhang, Xiaohui
Brody, Jerome S
Lenburg, Marc E
Spira, Avrum
author_facet Sridhar, Sriram
Schembri, Frank
Zeskind, Julie
Shah, Vishal
Gustafson, Adam M
Steiling, Katrina
Liu, Gang
Dumas, Yves-Martine
Zhang, Xiaohui
Brody, Jerome S
Lenburg, Marc E
Spira, Avrum
author_sort Sridhar, Sriram
collection PubMed
description BACKGROUND: Cigarette smoking is a leading cause of preventable death and a significant cause of lung cancer and chronic obstructive pulmonary disease. Prior studies have demonstrated that smoking creates a field of molecular injury throughout the airway epithelium exposed to cigarette smoke. We have previously characterized gene expression in the bronchial epithelium of never smokers and identified the gene expression changes that occur in the mainstem bronchus in response to smoking. In this study, we explored relationships in whole-genome gene expression between extrathorcic (buccal and nasal) and intrathoracic (bronchial) epithelium in healthy current and never smokers. RESULTS: Using genes that have been previously defined as being expressed in the bronchial airway of never smokers (the "normal airway transcriptome"), we found that bronchial and nasal epithelium from non-smokers were most similar in gene expression when compared to other epithelial and nonepithelial tissues, with several antioxidant, detoxification, and structural genes being highly expressed in both the bronchus and nose. Principle component analysis of previously defined smoking-induced genes from the bronchus suggested that smoking had a similar effect on gene expression in nasal epithelium. Gene set enrichment analysis demonstrated that this set of genes was also highly enriched among the genes most altered by smoking in both nasal and buccal epithelial samples. The expression of several detoxification genes was commonly altered by smoking in all three respiratory epithelial tissues, suggesting a common airway-wide response to tobacco exposure. CONCLUSION: Our findings support a relationship between gene expression in extra- and intrathoracic airway epithelial cells and extend the concept of a smoking-induced field of injury to epithelial cells that line the mouth and nose. This relationship could potentially be utilized to develop a non-invasive biomarker for tobacco exposure as well as a non-invasive screening or diagnostic tool providing information about individual susceptibility to smoking-induced lung diseases.
format Text
id pubmed-2435556
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-24355562008-06-24 Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium Sridhar, Sriram Schembri, Frank Zeskind, Julie Shah, Vishal Gustafson, Adam M Steiling, Katrina Liu, Gang Dumas, Yves-Martine Zhang, Xiaohui Brody, Jerome S Lenburg, Marc E Spira, Avrum BMC Genomics Research Article BACKGROUND: Cigarette smoking is a leading cause of preventable death and a significant cause of lung cancer and chronic obstructive pulmonary disease. Prior studies have demonstrated that smoking creates a field of molecular injury throughout the airway epithelium exposed to cigarette smoke. We have previously characterized gene expression in the bronchial epithelium of never smokers and identified the gene expression changes that occur in the mainstem bronchus in response to smoking. In this study, we explored relationships in whole-genome gene expression between extrathorcic (buccal and nasal) and intrathoracic (bronchial) epithelium in healthy current and never smokers. RESULTS: Using genes that have been previously defined as being expressed in the bronchial airway of never smokers (the "normal airway transcriptome"), we found that bronchial and nasal epithelium from non-smokers were most similar in gene expression when compared to other epithelial and nonepithelial tissues, with several antioxidant, detoxification, and structural genes being highly expressed in both the bronchus and nose. Principle component analysis of previously defined smoking-induced genes from the bronchus suggested that smoking had a similar effect on gene expression in nasal epithelium. Gene set enrichment analysis demonstrated that this set of genes was also highly enriched among the genes most altered by smoking in both nasal and buccal epithelial samples. The expression of several detoxification genes was commonly altered by smoking in all three respiratory epithelial tissues, suggesting a common airway-wide response to tobacco exposure. CONCLUSION: Our findings support a relationship between gene expression in extra- and intrathoracic airway epithelial cells and extend the concept of a smoking-induced field of injury to epithelial cells that line the mouth and nose. This relationship could potentially be utilized to develop a non-invasive biomarker for tobacco exposure as well as a non-invasive screening or diagnostic tool providing information about individual susceptibility to smoking-induced lung diseases. BioMed Central 2008-05-30 /pmc/articles/PMC2435556/ /pubmed/18513428 http://dx.doi.org/10.1186/1471-2164-9-259 Text en Copyright © 2008 Sridhar et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sridhar, Sriram
Schembri, Frank
Zeskind, Julie
Shah, Vishal
Gustafson, Adam M
Steiling, Katrina
Liu, Gang
Dumas, Yves-Martine
Zhang, Xiaohui
Brody, Jerome S
Lenburg, Marc E
Spira, Avrum
Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
title Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
title_full Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
title_fullStr Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
title_full_unstemmed Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
title_short Smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
title_sort smoking-induced gene expression changes in the bronchial airway are reflected in nasal and buccal epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435556/
https://www.ncbi.nlm.nih.gov/pubmed/18513428
http://dx.doi.org/10.1186/1471-2164-9-259
work_keys_str_mv AT sridharsriram smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT schembrifrank smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT zeskindjulie smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT shahvishal smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT gustafsonadamm smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT steilingkatrina smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT liugang smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT dumasyvesmartine smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT zhangxiaohui smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT brodyjeromes smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT lenburgmarce smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium
AT spiraavrum smokinginducedgeneexpressionchangesinthebronchialairwayarereflectedinnasalandbuccalepithelium