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Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer
BACKGROUND: We investigated the presence and distribution of the sentinel and the non-sentinel node micrometastases using complete serial sectioning and immunohistochemical staining (IHC), to inspect whether lymph node micrometastases spread to the sentinel lymph nodes first. METHODS: A total of 35...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2438334/ https://www.ncbi.nlm.nih.gov/pubmed/18577253 http://dx.doi.org/10.1186/1756-9966-27-7 |
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author | Ishii, Kaname Kinami, Shinichi Funaki, Kenichiro Fujita, Hideto Ninomiya, Itasu Fushida, Sachio Fujimura, Takashi Nishimura, Genichi Kayahara, Masato |
author_facet | Ishii, Kaname Kinami, Shinichi Funaki, Kenichiro Fujita, Hideto Ninomiya, Itasu Fushida, Sachio Fujimura, Takashi Nishimura, Genichi Kayahara, Masato |
author_sort | Ishii, Kaname |
collection | PubMed |
description | BACKGROUND: We investigated the presence and distribution of the sentinel and the non-sentinel node micrometastases using complete serial sectioning and immunohistochemical staining (IHC), to inspect whether lymph node micrometastases spread to the sentinel lymph nodes first. METHODS: A total of 35 patients, who underwent gastrectomy with a sentinel lymph node biopsy for gastric cancer, were enrolled in this study. Total of 1028 lymph nodes of 35 patients having gastric cancer without metastasis of lymph node by permanent section with hematoxylin and eosin staining (H&E) were selected. There were 252 sentinel nodes and the other 776 were non-sentinel nodes. All nodes were sectioned serially and stained alternately with H&E and IHC. Lymph node micrometastases was defined as proving to be positive first either the IHC or the complete serial sectioning. RESULTS: Micrometastases were detected in 4 (11%) of the 35 patients, 6 (0.58%) of 1028 nodes. Of these 4 patients, 3 had micrometastases exclusively in sentinel nodes, and the other had micrometastasis in both sentinel and non-sentinel nodes. There was no patient who had the micrometasitases only in non-sentinel nodes. CONCLUSION: These results support the concept that lymph node micrometastasis of gastric cancer spreads first to sentinel nodes. |
format | Text |
id | pubmed-2438334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-24383342008-06-25 Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer Ishii, Kaname Kinami, Shinichi Funaki, Kenichiro Fujita, Hideto Ninomiya, Itasu Fushida, Sachio Fujimura, Takashi Nishimura, Genichi Kayahara, Masato J Exp Clin Cancer Res Research BACKGROUND: We investigated the presence and distribution of the sentinel and the non-sentinel node micrometastases using complete serial sectioning and immunohistochemical staining (IHC), to inspect whether lymph node micrometastases spread to the sentinel lymph nodes first. METHODS: A total of 35 patients, who underwent gastrectomy with a sentinel lymph node biopsy for gastric cancer, were enrolled in this study. Total of 1028 lymph nodes of 35 patients having gastric cancer without metastasis of lymph node by permanent section with hematoxylin and eosin staining (H&E) were selected. There were 252 sentinel nodes and the other 776 were non-sentinel nodes. All nodes were sectioned serially and stained alternately with H&E and IHC. Lymph node micrometastases was defined as proving to be positive first either the IHC or the complete serial sectioning. RESULTS: Micrometastases were detected in 4 (11%) of the 35 patients, 6 (0.58%) of 1028 nodes. Of these 4 patients, 3 had micrometastases exclusively in sentinel nodes, and the other had micrometastasis in both sentinel and non-sentinel nodes. There was no patient who had the micrometasitases only in non-sentinel nodes. CONCLUSION: These results support the concept that lymph node micrometastasis of gastric cancer spreads first to sentinel nodes. BioMed Central 2008-05-30 /pmc/articles/PMC2438334/ /pubmed/18577253 http://dx.doi.org/10.1186/1756-9966-27-7 Text en Copyright © 2008 Ishii et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ishii, Kaname Kinami, Shinichi Funaki, Kenichiro Fujita, Hideto Ninomiya, Itasu Fushida, Sachio Fujimura, Takashi Nishimura, Genichi Kayahara, Masato Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
title | Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
title_full | Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
title_fullStr | Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
title_full_unstemmed | Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
title_short | Detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
title_sort | detection of sentinel and non-sentinel lymph node micrometastases by complete serial sectioning and immunohistochemical analysis for gastric cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2438334/ https://www.ncbi.nlm.nih.gov/pubmed/18577253 http://dx.doi.org/10.1186/1756-9966-27-7 |
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