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Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

BACKGROUND: Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic E. coli. R...

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Autores principales: Sidhu, Vishaldeep K, Vorhölter, Frank-Jörg, Niehaus, Karsten, Watt, Steven A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2438364/
https://www.ncbi.nlm.nih.gov/pubmed/18518965
http://dx.doi.org/10.1186/1471-2180-8-87
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author Sidhu, Vishaldeep K
Vorhölter, Frank-Jörg
Niehaus, Karsten
Watt, Steven A
author_facet Sidhu, Vishaldeep K
Vorhölter, Frank-Jörg
Niehaus, Karsten
Watt, Steven A
author_sort Sidhu, Vishaldeep K
collection PubMed
description BACKGROUND: Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic E. coli. RESULTS: We have demonstrated that Xanthomonas campestris pv. campestris (Xcc) releases OMVs into the culture supernatant during growth. A proteome study identified 31 different proteins that associate with the OMV fraction of which half are virulence-associated. A comparison with the most abundant outer membrane (OM) proteins revealed that some proteins are enriched in the OMV fraction. This may be connected to differences in the LPS composition between the OMVs and the OM. Furthermore, a comparison of the OMV proteomes from two different culture media indicated that the culture conditions have an impact on the protein composition. Interestingly, the proteins that are common to both culture conditions are mainly involved in virulence. CONCLUSION: Outer membrane vesicles released from the OM of Xcc contain membrane- and virulence-associated proteins. Future experiments will prove whether these structures can serve as "vehicles" for the transport of virulence factors into the host membrane.
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spelling pubmed-24383642008-06-26 Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris Sidhu, Vishaldeep K Vorhölter, Frank-Jörg Niehaus, Karsten Watt, Steven A BMC Microbiol Research Article BACKGROUND: Outer membrane vesicles (OMVs) are released from the outer membrane of many Gram-negative bacteria. These extracellular compartments are known to transport compounds involved in cell-cell signalling as well as virulence associated proteins, e.g. the cytolysine from enterotoxic E. coli. RESULTS: We have demonstrated that Xanthomonas campestris pv. campestris (Xcc) releases OMVs into the culture supernatant during growth. A proteome study identified 31 different proteins that associate with the OMV fraction of which half are virulence-associated. A comparison with the most abundant outer membrane (OM) proteins revealed that some proteins are enriched in the OMV fraction. This may be connected to differences in the LPS composition between the OMVs and the OM. Furthermore, a comparison of the OMV proteomes from two different culture media indicated that the culture conditions have an impact on the protein composition. Interestingly, the proteins that are common to both culture conditions are mainly involved in virulence. CONCLUSION: Outer membrane vesicles released from the OM of Xcc contain membrane- and virulence-associated proteins. Future experiments will prove whether these structures can serve as "vehicles" for the transport of virulence factors into the host membrane. BioMed Central 2008-06-02 /pmc/articles/PMC2438364/ /pubmed/18518965 http://dx.doi.org/10.1186/1471-2180-8-87 Text en Copyright © 2008 Sidhu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sidhu, Vishaldeep K
Vorhölter, Frank-Jörg
Niehaus, Karsten
Watt, Steven A
Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris
title Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris
title_full Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris
title_fullStr Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris
title_full_unstemmed Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris
title_short Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris
title_sort analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium xanthomonas campestris pv. campestris
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2438364/
https://www.ncbi.nlm.nih.gov/pubmed/18518965
http://dx.doi.org/10.1186/1471-2180-8-87
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