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The Recombinational Anatomy of a Mouse Chromosome

Among mammals, genetic recombination occurs at highly delimited sites known as recombination hotspots. They are typically 1–2 kb long and vary as much as a 1,000-fold or more in recombination activity. Although much is known about the molecular details of the recombination process itself, the factor...

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Autores principales: Paigen, Kenneth, Szatkiewicz, Jin P., Sawyer, Kathryn, Leahy, Nicole, Parvanov, Emil D., Ng, Siemon H. S., Graber, Joel H., Broman, Karl W., Petkov, Petko M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440539/
https://www.ncbi.nlm.nih.gov/pubmed/18617997
http://dx.doi.org/10.1371/journal.pgen.1000119
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author Paigen, Kenneth
Szatkiewicz, Jin P.
Sawyer, Kathryn
Leahy, Nicole
Parvanov, Emil D.
Ng, Siemon H. S.
Graber, Joel H.
Broman, Karl W.
Petkov, Petko M.
author_facet Paigen, Kenneth
Szatkiewicz, Jin P.
Sawyer, Kathryn
Leahy, Nicole
Parvanov, Emil D.
Ng, Siemon H. S.
Graber, Joel H.
Broman, Karl W.
Petkov, Petko M.
author_sort Paigen, Kenneth
collection PubMed
description Among mammals, genetic recombination occurs at highly delimited sites known as recombination hotspots. They are typically 1–2 kb long and vary as much as a 1,000-fold or more in recombination activity. Although much is known about the molecular details of the recombination process itself, the factors determining the location and relative activity of hotspots are poorly understood. To further our understanding, we have collected and mapped the locations of 5,472 crossover events along mouse Chromosome 1 arising in 6,028 meioses of male and female reciprocal F1 hybrids of C57BL/6J and CAST/EiJ mice. Crossovers were mapped to a minimum resolution of 225 kb, and those in the telomere-proximal 24.7 Mb were further mapped to resolve individual hotspots. Recombination rates were evolutionarily conserved on a regional scale, but not at the local level. There was a clear negative-exponential relationship between the relative activity and abundance of hotspot activity classes, such that a small number of the most active hotspots account for the majority of recombination. Females had 1.2× higher overall recombination than males did, although the sex ratio showed considerable regional variation. Locally, entirely sex-specific hotspots were rare. The initiation of recombination at the most active hotspot was regulated independently on the two parental chromatids, and analysis of reciprocal crosses indicated that parental imprinting has subtle effects on recombination rates. It appears that the regulation of mammalian recombination is a complex, dynamic process involving multiple factors reflecting species, sex, individual variation within species, and the properties of individual hotspots.
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spelling pubmed-24405392008-07-11 The Recombinational Anatomy of a Mouse Chromosome Paigen, Kenneth Szatkiewicz, Jin P. Sawyer, Kathryn Leahy, Nicole Parvanov, Emil D. Ng, Siemon H. S. Graber, Joel H. Broman, Karl W. Petkov, Petko M. PLoS Genet Research Article Among mammals, genetic recombination occurs at highly delimited sites known as recombination hotspots. They are typically 1–2 kb long and vary as much as a 1,000-fold or more in recombination activity. Although much is known about the molecular details of the recombination process itself, the factors determining the location and relative activity of hotspots are poorly understood. To further our understanding, we have collected and mapped the locations of 5,472 crossover events along mouse Chromosome 1 arising in 6,028 meioses of male and female reciprocal F1 hybrids of C57BL/6J and CAST/EiJ mice. Crossovers were mapped to a minimum resolution of 225 kb, and those in the telomere-proximal 24.7 Mb were further mapped to resolve individual hotspots. Recombination rates were evolutionarily conserved on a regional scale, but not at the local level. There was a clear negative-exponential relationship between the relative activity and abundance of hotspot activity classes, such that a small number of the most active hotspots account for the majority of recombination. Females had 1.2× higher overall recombination than males did, although the sex ratio showed considerable regional variation. Locally, entirely sex-specific hotspots were rare. The initiation of recombination at the most active hotspot was regulated independently on the two parental chromatids, and analysis of reciprocal crosses indicated that parental imprinting has subtle effects on recombination rates. It appears that the regulation of mammalian recombination is a complex, dynamic process involving multiple factors reflecting species, sex, individual variation within species, and the properties of individual hotspots. Public Library of Science 2008-07-11 /pmc/articles/PMC2440539/ /pubmed/18617997 http://dx.doi.org/10.1371/journal.pgen.1000119 Text en Paigen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Paigen, Kenneth
Szatkiewicz, Jin P.
Sawyer, Kathryn
Leahy, Nicole
Parvanov, Emil D.
Ng, Siemon H. S.
Graber, Joel H.
Broman, Karl W.
Petkov, Petko M.
The Recombinational Anatomy of a Mouse Chromosome
title The Recombinational Anatomy of a Mouse Chromosome
title_full The Recombinational Anatomy of a Mouse Chromosome
title_fullStr The Recombinational Anatomy of a Mouse Chromosome
title_full_unstemmed The Recombinational Anatomy of a Mouse Chromosome
title_short The Recombinational Anatomy of a Mouse Chromosome
title_sort recombinational anatomy of a mouse chromosome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440539/
https://www.ncbi.nlm.nih.gov/pubmed/18617997
http://dx.doi.org/10.1371/journal.pgen.1000119
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