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Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus

BACKGROUND: Natural contamination and anthropogenic pollution of soils are likely to be major determinants of functioning and survival of keystone invertebrate taxa. Soil animals will have both evolutionary adaptation and genetically programmed responses to these toxic chemicals, but mechanistic und...

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Autores principales: Owen, Jennifer, Hedley, B Ann, Svendsen, Claus, Wren, Jodie, Jonker, Martijs J, Hankard, Peter K, Lister, Linsey J, Stürzenbaum, Stephen R, Morgan, A John, Spurgeon, David J, Blaxter, Mark L, Kille, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440553/
https://www.ncbi.nlm.nih.gov/pubmed/18522720
http://dx.doi.org/10.1186/1471-2164-9-266
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author Owen, Jennifer
Hedley, B Ann
Svendsen, Claus
Wren, Jodie
Jonker, Martijs J
Hankard, Peter K
Lister, Linsey J
Stürzenbaum, Stephen R
Morgan, A John
Spurgeon, David J
Blaxter, Mark L
Kille, Peter
author_facet Owen, Jennifer
Hedley, B Ann
Svendsen, Claus
Wren, Jodie
Jonker, Martijs J
Hankard, Peter K
Lister, Linsey J
Stürzenbaum, Stephen R
Morgan, A John
Spurgeon, David J
Blaxter, Mark L
Kille, Peter
author_sort Owen, Jennifer
collection PubMed
description BACKGROUND: Natural contamination and anthropogenic pollution of soils are likely to be major determinants of functioning and survival of keystone invertebrate taxa. Soil animals will have both evolutionary adaptation and genetically programmed responses to these toxic chemicals, but mechanistic understanding of such is sparse. The clitellate annelid Lumbricus rubellus is a model organism for soil health testing, but genetic data have been lacking. RESULTS: We generated a 17,000 sequence expressed sequence tag dataset, defining ~8,100 different putative genes, and built an 8,000-element transcriptome microarray for L. rubellus. Strikingly, less than half the putative genes (43%) were assigned annotations from the gene ontology (GO) system; this reflects the phylogenetic uniqueness of earthworms compared to the well-annotated model animals. The microarray was used to identify adult- and juvenile-specific transcript profiles in untreated animals and to determine dose-response transcription profiles following exposure to three xenobiotics from different chemical classes: inorganic (the metal cadmium), organic (the polycyclic aromatic hydrocarbon fluoranthene), and agrochemical (the herbicide atrazine). Analysis of these profiles revealed compound-specific fingerprints which identify the molecular responses of this annelid to each contaminant. The data and analyses are available in an integrated database, LumbriBASE. CONCLUSION: L. rubellus has a complex response to contaminant exposure, but this can be efficiently analysed using molecular methods, revealing unique response profiles for different classes of effector. These profiles may assist in the development of novel monitoring or bioremediation protocols, as well as in understanding the ecosystem effects of exposure.
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spelling pubmed-24405532008-06-27 Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus Owen, Jennifer Hedley, B Ann Svendsen, Claus Wren, Jodie Jonker, Martijs J Hankard, Peter K Lister, Linsey J Stürzenbaum, Stephen R Morgan, A John Spurgeon, David J Blaxter, Mark L Kille, Peter BMC Genomics Research Article BACKGROUND: Natural contamination and anthropogenic pollution of soils are likely to be major determinants of functioning and survival of keystone invertebrate taxa. Soil animals will have both evolutionary adaptation and genetically programmed responses to these toxic chemicals, but mechanistic understanding of such is sparse. The clitellate annelid Lumbricus rubellus is a model organism for soil health testing, but genetic data have been lacking. RESULTS: We generated a 17,000 sequence expressed sequence tag dataset, defining ~8,100 different putative genes, and built an 8,000-element transcriptome microarray for L. rubellus. Strikingly, less than half the putative genes (43%) were assigned annotations from the gene ontology (GO) system; this reflects the phylogenetic uniqueness of earthworms compared to the well-annotated model animals. The microarray was used to identify adult- and juvenile-specific transcript profiles in untreated animals and to determine dose-response transcription profiles following exposure to three xenobiotics from different chemical classes: inorganic (the metal cadmium), organic (the polycyclic aromatic hydrocarbon fluoranthene), and agrochemical (the herbicide atrazine). Analysis of these profiles revealed compound-specific fingerprints which identify the molecular responses of this annelid to each contaminant. The data and analyses are available in an integrated database, LumbriBASE. CONCLUSION: L. rubellus has a complex response to contaminant exposure, but this can be efficiently analysed using molecular methods, revealing unique response profiles for different classes of effector. These profiles may assist in the development of novel monitoring or bioremediation protocols, as well as in understanding the ecosystem effects of exposure. BioMed Central 2008-06-03 /pmc/articles/PMC2440553/ /pubmed/18522720 http://dx.doi.org/10.1186/1471-2164-9-266 Text en Copyright © 2008 Owen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Owen, Jennifer
Hedley, B Ann
Svendsen, Claus
Wren, Jodie
Jonker, Martijs J
Hankard, Peter K
Lister, Linsey J
Stürzenbaum, Stephen R
Morgan, A John
Spurgeon, David J
Blaxter, Mark L
Kille, Peter
Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
title Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
title_full Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
title_fullStr Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
title_full_unstemmed Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
title_short Transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid Lumbricus rubellus
title_sort transcriptome profiling of developmental and xenobiotic responses in a keystone soil animal, the oligochaete annelid lumbricus rubellus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440553/
https://www.ncbi.nlm.nih.gov/pubmed/18522720
http://dx.doi.org/10.1186/1471-2164-9-266
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