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btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe

btn1, the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3, exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37°C that culminate...

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Detalles Bibliográficos
Autores principales: Codlin, Sandra, Haines, Rebecca L, Mole, Sara E
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440566/
https://www.ncbi.nlm.nih.gov/pubmed/18346214
http://dx.doi.org/10.1111/j.1600-0854.2008.00735.x
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author Codlin, Sandra
Haines, Rebecca L
Mole, Sara E
author_facet Codlin, Sandra
Haines, Rebecca L
Mole, Sara E
author_sort Codlin, Sandra
collection PubMed
description btn1, the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3, exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37°C that culminate in total depolarized growth and cell lysis. These defects are preceded by a progressive failure to correctly polarize sterol-rich domains after cytokinesis and are accompanied by loss of Myo1p localization. Furthermore, we found that in Sz. pombe, sterol spreading is linked to defective formation/polarization of F-actin patches and disruption of endocytosis and that these processes are aberrant in btn1Δ cells. Consistent with a role for Btn1p in polarized growth, Btn1p has an altered location at 37°C and is retained in actin-dependent endomembrane structures near the cell poles or septum.
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spelling pubmed-24405662008-07-01 btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe Codlin, Sandra Haines, Rebecca L Mole, Sara E Traffic Original Articles btn1, the Schizosaccharomyces pombe orthologue of the human Batten disease gene CLN3, exerts multiple cellular effects. As well as a role in vacuole pH homoeostasis, we now show that Btn1p is essential for growth at high temperatures. Its absence results in progressive defects at 37°C that culminate in total depolarized growth and cell lysis. These defects are preceded by a progressive failure to correctly polarize sterol-rich domains after cytokinesis and are accompanied by loss of Myo1p localization. Furthermore, we found that in Sz. pombe, sterol spreading is linked to defective formation/polarization of F-actin patches and disruption of endocytosis and that these processes are aberrant in btn1Δ cells. Consistent with a role for Btn1p in polarized growth, Btn1p has an altered location at 37°C and is retained in actin-dependent endomembrane structures near the cell poles or septum. Blackwell Publishing Ltd 2008-06 /pmc/articles/PMC2440566/ /pubmed/18346214 http://dx.doi.org/10.1111/j.1600-0854.2008.00735.x Text en © 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd
spellingShingle Original Articles
Codlin, Sandra
Haines, Rebecca L
Mole, Sara E
btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
title btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
title_full btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
title_fullStr btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
title_full_unstemmed btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
title_short btn1 Affects Endocytosis, Polarization of Sterol-Rich Membrane Domains and Polarized Growth in Schizosaccharomyces pombe
title_sort btn1 affects endocytosis, polarization of sterol-rich membrane domains and polarized growth in schizosaccharomyces pombe
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440566/
https://www.ncbi.nlm.nih.gov/pubmed/18346214
http://dx.doi.org/10.1111/j.1600-0854.2008.00735.x
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