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Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags

BACKGROUND: The bite of spiders belonging to the genus Loxosceles can induce a variety of clinical symptoms, including dermonecrosis, thrombosis, vascular leakage, haemolysis, and persistent inflammation. In order to examine the transcripts expressed in venom gland of Loxosceles laeta spider and to...

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Autores principales: Fernandes-Pedrosa, Matheus de F, Junqueira-de-Azevedo, Inácio de LM, Gonçalves-de-Andrade, Rute M, Kobashi, Leonardo S, Almeida, Diego D, Ho, Paulo L, Tambourgi, Denise V
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440767/
https://www.ncbi.nlm.nih.gov/pubmed/18547439
http://dx.doi.org/10.1186/1471-2164-9-279
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author Fernandes-Pedrosa, Matheus de F
Junqueira-de-Azevedo, Inácio de LM
Gonçalves-de-Andrade, Rute M
Kobashi, Leonardo S
Almeida, Diego D
Ho, Paulo L
Tambourgi, Denise V
author_facet Fernandes-Pedrosa, Matheus de F
Junqueira-de-Azevedo, Inácio de LM
Gonçalves-de-Andrade, Rute M
Kobashi, Leonardo S
Almeida, Diego D
Ho, Paulo L
Tambourgi, Denise V
author_sort Fernandes-Pedrosa, Matheus de F
collection PubMed
description BACKGROUND: The bite of spiders belonging to the genus Loxosceles can induce a variety of clinical symptoms, including dermonecrosis, thrombosis, vascular leakage, haemolysis, and persistent inflammation. In order to examine the transcripts expressed in venom gland of Loxosceles laeta spider and to unveil the potential of its products on cellular structure and functional aspects, we generated 3,008 expressed sequence tags (ESTs) from a cDNA library. RESULTS: All ESTs were clustered into 1,357 clusters, of which 16.4% of the total ESTs belong to recognized toxin-coding sequences, being the Sphingomyelinases D the most abundant transcript; 14.5% include "possible toxins", whose transcripts correspond to metalloproteinases, serinoproteinases, hyaluronidases, lipases, C-lectins, cystein peptidases and inhibitors. Thirty three percent of the ESTs are similar to cellular transcripts, being the major part represented by molecules involved in gene and protein expression, reflecting the specialization of this tissue for protein synthesis. In addition, a considerable number of sequences, 25%, has no significant similarity to any known sequence. CONCLUSION: This study provides a first global view of the gene expression scenario of the venom gland of L. laeta described so far, indicating the molecular bases of its venom composition.
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spelling pubmed-24407672008-06-27 Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags Fernandes-Pedrosa, Matheus de F Junqueira-de-Azevedo, Inácio de LM Gonçalves-de-Andrade, Rute M Kobashi, Leonardo S Almeida, Diego D Ho, Paulo L Tambourgi, Denise V BMC Genomics Research Article BACKGROUND: The bite of spiders belonging to the genus Loxosceles can induce a variety of clinical symptoms, including dermonecrosis, thrombosis, vascular leakage, haemolysis, and persistent inflammation. In order to examine the transcripts expressed in venom gland of Loxosceles laeta spider and to unveil the potential of its products on cellular structure and functional aspects, we generated 3,008 expressed sequence tags (ESTs) from a cDNA library. RESULTS: All ESTs were clustered into 1,357 clusters, of which 16.4% of the total ESTs belong to recognized toxin-coding sequences, being the Sphingomyelinases D the most abundant transcript; 14.5% include "possible toxins", whose transcripts correspond to metalloproteinases, serinoproteinases, hyaluronidases, lipases, C-lectins, cystein peptidases and inhibitors. Thirty three percent of the ESTs are similar to cellular transcripts, being the major part represented by molecules involved in gene and protein expression, reflecting the specialization of this tissue for protein synthesis. In addition, a considerable number of sequences, 25%, has no significant similarity to any known sequence. CONCLUSION: This study provides a first global view of the gene expression scenario of the venom gland of L. laeta described so far, indicating the molecular bases of its venom composition. BioMed Central 2008-06-12 /pmc/articles/PMC2440767/ /pubmed/18547439 http://dx.doi.org/10.1186/1471-2164-9-279 Text en Copyright © 2008 Fernandes-Pedrosa et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fernandes-Pedrosa, Matheus de F
Junqueira-de-Azevedo, Inácio de LM
Gonçalves-de-Andrade, Rute M
Kobashi, Leonardo S
Almeida, Diego D
Ho, Paulo L
Tambourgi, Denise V
Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags
title Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags
title_full Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags
title_fullStr Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags
title_full_unstemmed Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags
title_short Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags
title_sort transcriptome analysis of loxosceles laeta (araneae, sicariidae) spider venomous gland using expressed sequence tags
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2440767/
https://www.ncbi.nlm.nih.gov/pubmed/18547439
http://dx.doi.org/10.1186/1471-2164-9-279
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