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DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference

[Fe(2)L(3)](4+) (L = C(25)H(20)N(4)) is a synthetic tetracationic supramolecular cylinder (with a triple helical architecture) that targets the major groove of DNA and can bind to DNA Y-shaped junctions. To explore the DNA-binding mode of [Fe(2)L(3)](4+), we examine herein the interactions of pure e...

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Autores principales: Malina, Jaroslav, Hannon, Michael J., Brabec, Viktor
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2441793/
https://www.ncbi.nlm.nih.gov/pubmed/18467423
http://dx.doi.org/10.1093/nar/gkn244
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author Malina, Jaroslav
Hannon, Michael J.
Brabec, Viktor
author_facet Malina, Jaroslav
Hannon, Michael J.
Brabec, Viktor
author_sort Malina, Jaroslav
collection PubMed
description [Fe(2)L(3)](4+) (L = C(25)H(20)N(4)) is a synthetic tetracationic supramolecular cylinder (with a triple helical architecture) that targets the major groove of DNA and can bind to DNA Y-shaped junctions. To explore the DNA-binding mode of [Fe(2)L(3)](4+), we examine herein the interactions of pure enantiomers of this cylinder with DNA by biochemical and molecular biology methods. The results have revealed that, in addition to the previously reported bending of DNA, the enantiomers extensively unwind DNA, with the M enantiomer being the more efficient at unwinding, and exhibit preferential binding to regular alternating purine–pyrimidine sequences, with the M enantiomer showing a greater preference. Also, interestingly, the DNA binding of bulky cylinders [Fe(2)(L-CF(3))(3)](4+) and [Fe(2)(L-Ph)(3)](4+) results in no DNA unwinding and also no sequence preference of their DNA binding was observed. The observation of sequence-preference in the binding of these supramolecular cylinders suggests that a concept based on the use of metallosupramolecular cylinders might result in molecular designs that recognize the genetic code in a sequence-dependent manner with a potential ability to affect the processing of the genetic code.
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spelling pubmed-24417932008-07-02 DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference Malina, Jaroslav Hannon, Michael J. Brabec, Viktor Nucleic Acids Res Chemistry [Fe(2)L(3)](4+) (L = C(25)H(20)N(4)) is a synthetic tetracationic supramolecular cylinder (with a triple helical architecture) that targets the major groove of DNA and can bind to DNA Y-shaped junctions. To explore the DNA-binding mode of [Fe(2)L(3)](4+), we examine herein the interactions of pure enantiomers of this cylinder with DNA by biochemical and molecular biology methods. The results have revealed that, in addition to the previously reported bending of DNA, the enantiomers extensively unwind DNA, with the M enantiomer being the more efficient at unwinding, and exhibit preferential binding to regular alternating purine–pyrimidine sequences, with the M enantiomer showing a greater preference. Also, interestingly, the DNA binding of bulky cylinders [Fe(2)(L-CF(3))(3)](4+) and [Fe(2)(L-Ph)(3)](4+) results in no DNA unwinding and also no sequence preference of their DNA binding was observed. The observation of sequence-preference in the binding of these supramolecular cylinders suggests that a concept based on the use of metallosupramolecular cylinders might result in molecular designs that recognize the genetic code in a sequence-dependent manner with a potential ability to affect the processing of the genetic code. Oxford University Press 2008-06 2008-05-08 /pmc/articles/PMC2441793/ /pubmed/18467423 http://dx.doi.org/10.1093/nar/gkn244 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Malina, Jaroslav
Hannon, Michael J.
Brabec, Viktor
DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
title DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
title_full DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
title_fullStr DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
title_full_unstemmed DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
title_short DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
title_sort dna binding of dinuclear iron(ii) metallosupramolecular cylinders. dna unwinding and sequence preference
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2441793/
https://www.ncbi.nlm.nih.gov/pubmed/18467423
http://dx.doi.org/10.1093/nar/gkn244
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