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β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients

Colorectal cancer (CRC) is the fourth most common non-cutaneous malignancy in the United States and the second most frequent cause of cancer-related death. One of the most important determinants of CRC survival is lymph node metastasis. To determine whether molecular markers might be prognostic for...

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Autores principales: Shrout, J, Yousefzadeh, M, Dodd, A, Kirven, K, Blum, C, Graham, A, Benjamin, K, Hoda, R, Krishna, M, Romano, M, Wallace, M, Garrett-Mayer, E, Mitas, M
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2441949/
https://www.ncbi.nlm.nih.gov/pubmed/18506145
http://dx.doi.org/10.1038/sj.bjc.6604399
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author Shrout, J
Yousefzadeh, M
Dodd, A
Kirven, K
Blum, C
Graham, A
Benjamin, K
Hoda, R
Krishna, M
Romano, M
Wallace, M
Garrett-Mayer, E
Mitas, M
author_facet Shrout, J
Yousefzadeh, M
Dodd, A
Kirven, K
Blum, C
Graham, A
Benjamin, K
Hoda, R
Krishna, M
Romano, M
Wallace, M
Garrett-Mayer, E
Mitas, M
author_sort Shrout, J
collection PubMed
description Colorectal cancer (CRC) is the fourth most common non-cutaneous malignancy in the United States and the second most frequent cause of cancer-related death. One of the most important determinants of CRC survival is lymph node metastasis. To determine whether molecular markers might be prognostic for lymph node metastases, we measured by quantitative real-time RT–PCR the expression levels of 15 cancer-associated genes in formalin-fixed paraffin-embedded primary tissues derived from stage I–IV CRC patients with (n=20) and without (n=18) nodal metastases. Using the mean of the 15 genes as an internal reference control, we observed that low expression of β(2)microglobulin (B2M) was a strong prognostic indicator of lymph node metastases (area under the curve (AUC)=0.85; 95% confidence interval (CI)=0.69–0.94). We also observed that the expression ratio of B2M/Spint2 had the highest prognostic accuracy (AUC=0.87; 95% CI=0.71–0.96) of all potential two-gene combinations. Expression values of Spint2 correlated with the mean of the entire gene set at an R(2) value of 0.97, providing evidence that Spint2 serves not as an independent prognostic gene, but rather as a reliable reference control gene. These studies are the first to demonstrate a prognostic role of B2M at the mRNA level and suggest that low B2M expression levels might be useful for identifying patients with lymph node metastasis and/or poor survival.
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spelling pubmed-24419492009-09-10 β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients Shrout, J Yousefzadeh, M Dodd, A Kirven, K Blum, C Graham, A Benjamin, K Hoda, R Krishna, M Romano, M Wallace, M Garrett-Mayer, E Mitas, M Br J Cancer Molecular Diagnostics Colorectal cancer (CRC) is the fourth most common non-cutaneous malignancy in the United States and the second most frequent cause of cancer-related death. One of the most important determinants of CRC survival is lymph node metastasis. To determine whether molecular markers might be prognostic for lymph node metastases, we measured by quantitative real-time RT–PCR the expression levels of 15 cancer-associated genes in formalin-fixed paraffin-embedded primary tissues derived from stage I–IV CRC patients with (n=20) and without (n=18) nodal metastases. Using the mean of the 15 genes as an internal reference control, we observed that low expression of β(2)microglobulin (B2M) was a strong prognostic indicator of lymph node metastases (area under the curve (AUC)=0.85; 95% confidence interval (CI)=0.69–0.94). We also observed that the expression ratio of B2M/Spint2 had the highest prognostic accuracy (AUC=0.87; 95% CI=0.71–0.96) of all potential two-gene combinations. Expression values of Spint2 correlated with the mean of the entire gene set at an R(2) value of 0.97, providing evidence that Spint2 serves not as an independent prognostic gene, but rather as a reliable reference control gene. These studies are the first to demonstrate a prognostic role of B2M at the mRNA level and suggest that low B2M expression levels might be useful for identifying patients with lymph node metastasis and/or poor survival. Nature Publishing Group 2008-06-17 2008-05-27 /pmc/articles/PMC2441949/ /pubmed/18506145 http://dx.doi.org/10.1038/sj.bjc.6604399 Text en Copyright © 2008 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular Diagnostics
Shrout, J
Yousefzadeh, M
Dodd, A
Kirven, K
Blum, C
Graham, A
Benjamin, K
Hoda, R
Krishna, M
Romano, M
Wallace, M
Garrett-Mayer, E
Mitas, M
β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
title β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
title_full β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
title_fullStr β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
title_full_unstemmed β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
title_short β(2)microglobulin mRNA expression levels are prognostic for lymph node metastasis in colorectal cancer patients
title_sort β(2)microglobulin mrna expression levels are prognostic for lymph node metastasis in colorectal cancer patients
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2441949/
https://www.ncbi.nlm.nih.gov/pubmed/18506145
http://dx.doi.org/10.1038/sj.bjc.6604399
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