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Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth

The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the p...

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Detalles Bibliográficos
Autores principales: Lee, Yong Jik, Szumlanski, Amy, Nielsen, Erik, Yang, Zhenbiao
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442199/
https://www.ncbi.nlm.nih.gov/pubmed/18591430
http://dx.doi.org/10.1083/jcb.200801086
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author Lee, Yong Jik
Szumlanski, Amy
Nielsen, Erik
Yang, Zhenbiao
author_facet Lee, Yong Jik
Szumlanski, Amy
Nielsen, Erik
Yang, Zhenbiao
author_sort Lee, Yong Jik
collection PubMed
description The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis.
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spelling pubmed-24421992008-12-30 Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth Lee, Yong Jik Szumlanski, Amy Nielsen, Erik Yang, Zhenbiao J Cell Biol Research Articles The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis. The Rockefeller University Press 2008-06-30 /pmc/articles/PMC2442199/ /pubmed/18591430 http://dx.doi.org/10.1083/jcb.200801086 Text en © 2008 Lee et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Lee, Yong Jik
Szumlanski, Amy
Nielsen, Erik
Yang, Zhenbiao
Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
title Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
title_full Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
title_fullStr Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
title_full_unstemmed Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
title_short Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
title_sort rho-gtpase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442199/
https://www.ncbi.nlm.nih.gov/pubmed/18591430
http://dx.doi.org/10.1083/jcb.200801086
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