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Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the p...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442199/ https://www.ncbi.nlm.nih.gov/pubmed/18591430 http://dx.doi.org/10.1083/jcb.200801086 |
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author | Lee, Yong Jik Szumlanski, Amy Nielsen, Erik Yang, Zhenbiao |
author_facet | Lee, Yong Jik Szumlanski, Amy Nielsen, Erik Yang, Zhenbiao |
author_sort | Lee, Yong Jik |
collection | PubMed |
description | The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis. |
format | Text |
id | pubmed-2442199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-24421992008-12-30 Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth Lee, Yong Jik Szumlanski, Amy Nielsen, Erik Yang, Zhenbiao J Cell Biol Research Articles The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis. The Rockefeller University Press 2008-06-30 /pmc/articles/PMC2442199/ /pubmed/18591430 http://dx.doi.org/10.1083/jcb.200801086 Text en © 2008 Lee et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Lee, Yong Jik Szumlanski, Amy Nielsen, Erik Yang, Zhenbiao Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
title | Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
title_full | Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
title_fullStr | Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
title_full_unstemmed | Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
title_short | Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
title_sort | rho-gtpase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442199/ https://www.ncbi.nlm.nih.gov/pubmed/18591430 http://dx.doi.org/10.1083/jcb.200801086 |
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