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Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis

Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lap...

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Detalles Bibliográficos
Autores principales: Goss, John W., Toomre, Derek K.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442215/
https://www.ncbi.nlm.nih.gov/pubmed/18573914
http://dx.doi.org/10.1083/jcb.200712137
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author Goss, John W.
Toomre, Derek K.
author_facet Goss, John W.
Toomre, Derek K.
author_sort Goss, John W.
collection PubMed
description Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein–yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microscopy imaging provided direct evidence that Golgi-derived vesicles from both daughter cells not only traffic to the furrow region but dock and fuse there, supporting a symmetrically polarized exocytic delivery model. In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cytokinesis is composed of both symmetrical and asymmetrical stages.
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spelling pubmed-24422152008-12-30 Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis Goss, John W. Toomre, Derek K. J Cell Biol Research Articles Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein–yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microscopy imaging provided direct evidence that Golgi-derived vesicles from both daughter cells not only traffic to the furrow region but dock and fuse there, supporting a symmetrically polarized exocytic delivery model. In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cytokinesis is composed of both symmetrical and asymmetrical stages. The Rockefeller University Press 2008-06-30 /pmc/articles/PMC2442215/ /pubmed/18573914 http://dx.doi.org/10.1083/jcb.200712137 Text en © 2008 Goss and Toomre This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Goss, John W.
Toomre, Derek K.
Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
title Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
title_full Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
title_fullStr Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
title_full_unstemmed Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
title_short Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
title_sort both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442215/
https://www.ncbi.nlm.nih.gov/pubmed/18573914
http://dx.doi.org/10.1083/jcb.200712137
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