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Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis
Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lap...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442215/ https://www.ncbi.nlm.nih.gov/pubmed/18573914 http://dx.doi.org/10.1083/jcb.200712137 |
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author | Goss, John W. Toomre, Derek K. |
author_facet | Goss, John W. Toomre, Derek K. |
author_sort | Goss, John W. |
collection | PubMed |
description | Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein–yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microscopy imaging provided direct evidence that Golgi-derived vesicles from both daughter cells not only traffic to the furrow region but dock and fuse there, supporting a symmetrically polarized exocytic delivery model. In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cytokinesis is composed of both symmetrical and asymmetrical stages. |
format | Text |
id | pubmed-2442215 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-24422152008-12-30 Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis Goss, John W. Toomre, Derek K. J Cell Biol Research Articles Membrane trafficking during cytokinesis is not well understood. We used advanced live cell imaging techniques to track exocytosis of single vesicles to determine whether constitutively exocytosed membrane is focally delivered to the cleavage furrow. Ultrasensitive three-dimensional confocal time-lapse imaging of the temperature-sensitive membrane cargo protein vesicular stomatitis virus protein–yellow fluorescent protein revealed that vesicles from both daughter cells traffic out of the Golgi and into the furrow, following curvilinear paths. Immunolocalization and photobleaching experiments indicate that individual vesicles accumulate at the midbody and generate a reserve vesicle pool that is distinct from endosomal and lysosomal compartments. Total internal reflection fluorescence microscopy imaging provided direct evidence that Golgi-derived vesicles from both daughter cells not only traffic to the furrow region but dock and fuse there, supporting a symmetrically polarized exocytic delivery model. In contrast, quantitative analysis of midbody abscission showed inheritance of the midbody remnant by one daughter cell, indicating that cytokinesis is composed of both symmetrical and asymmetrical stages. The Rockefeller University Press 2008-06-30 /pmc/articles/PMC2442215/ /pubmed/18573914 http://dx.doi.org/10.1083/jcb.200712137 Text en © 2008 Goss and Toomre This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Goss, John W. Toomre, Derek K. Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
title | Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
title_full | Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
title_fullStr | Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
title_full_unstemmed | Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
title_short | Both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
title_sort | both daughter cells traffic and exocytose membrane at the cleavage furrow during mammalian cytokinesis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442215/ https://www.ncbi.nlm.nih.gov/pubmed/18573914 http://dx.doi.org/10.1083/jcb.200712137 |
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