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Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer

BACKGROUND: Viscum album (VA) preparations have been used as a complimentary therapy in cancer. In addition to their cytotoxic properties, they have also been shown to have immunostimulatory properties. In the present study, we examine the hypothesis that the VA preparations induce activation of hum...

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Autores principales: Elluru, Sri Ramulu, van Huyen, Jean-Paul Duong, Delignat, Sandrine, Kazatchkine, Michel D, Friboulet, Alain, Kaveri, Srini V, Bayry, Jagadeesh
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442603/
https://www.ncbi.nlm.nih.gov/pubmed/18533025
http://dx.doi.org/10.1186/1471-2407-8-161
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author Elluru, Sri Ramulu
van Huyen, Jean-Paul Duong
Delignat, Sandrine
Kazatchkine, Michel D
Friboulet, Alain
Kaveri, Srini V
Bayry, Jagadeesh
author_facet Elluru, Sri Ramulu
van Huyen, Jean-Paul Duong
Delignat, Sandrine
Kazatchkine, Michel D
Friboulet, Alain
Kaveri, Srini V
Bayry, Jagadeesh
author_sort Elluru, Sri Ramulu
collection PubMed
description BACKGROUND: Viscum album (VA) preparations have been used as a complimentary therapy in cancer. In addition to their cytotoxic properties, they have also been shown to have immunostimulatory properties. In the present study, we examine the hypothesis that the VA preparations induce activation of human DC that facilitates effective tumor regression. METHODS: Four day old monocyte-derived immature DCs were treated with VA Qu Spez at 5, 10 and 15 μg/ml for 48 hrs. The expression of surface molecules was analyzed by flow cytometry. The ability of Qu Spez-educated DC to stimulate T cells was analyzed by allogeneic mixed lymphocyte reaction and activation of Melan-A/MART-1-specific M77-80 CD8+T cells. Cytokines in cell free culture supernatant was analyzed by cytokine bead array assay. RESULTS: VA Qu Spez stimulated DCs presented with increased expression of antigen presenting molecule HLA-DR and of co-stimulatory molecules CD40, CD80 and CD86. The VA Qu Spez also induced the secretion of inflammatory cytokines IL-6 and IL-8. Further, Qu Spez-educated DC stimulated CD4+T cells in a allogeneic mixed lymphocyte reaction and activated melanoma antigen Melan-A/MART-1-specific M77-80 CD8+T cells as evidenced by increased secretion of TNF-α and IFNγ. CONCLUSION: The VA preparations stimulate the maturation and activation of human DCs, which may facilitate anti-tumoral immune responses. These results should assist in understanding the immunostimulatory properties of VA preparations and improving the therapeutic strategies.
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spelling pubmed-24426032008-07-02 Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer Elluru, Sri Ramulu van Huyen, Jean-Paul Duong Delignat, Sandrine Kazatchkine, Michel D Friboulet, Alain Kaveri, Srini V Bayry, Jagadeesh BMC Cancer Research Article BACKGROUND: Viscum album (VA) preparations have been used as a complimentary therapy in cancer. In addition to their cytotoxic properties, they have also been shown to have immunostimulatory properties. In the present study, we examine the hypothesis that the VA preparations induce activation of human DC that facilitates effective tumor regression. METHODS: Four day old monocyte-derived immature DCs were treated with VA Qu Spez at 5, 10 and 15 μg/ml for 48 hrs. The expression of surface molecules was analyzed by flow cytometry. The ability of Qu Spez-educated DC to stimulate T cells was analyzed by allogeneic mixed lymphocyte reaction and activation of Melan-A/MART-1-specific M77-80 CD8+T cells. Cytokines in cell free culture supernatant was analyzed by cytokine bead array assay. RESULTS: VA Qu Spez stimulated DCs presented with increased expression of antigen presenting molecule HLA-DR and of co-stimulatory molecules CD40, CD80 and CD86. The VA Qu Spez also induced the secretion of inflammatory cytokines IL-6 and IL-8. Further, Qu Spez-educated DC stimulated CD4+T cells in a allogeneic mixed lymphocyte reaction and activated melanoma antigen Melan-A/MART-1-specific M77-80 CD8+T cells as evidenced by increased secretion of TNF-α and IFNγ. CONCLUSION: The VA preparations stimulate the maturation and activation of human DCs, which may facilitate anti-tumoral immune responses. These results should assist in understanding the immunostimulatory properties of VA preparations and improving the therapeutic strategies. BioMed Central 2008-06-04 /pmc/articles/PMC2442603/ /pubmed/18533025 http://dx.doi.org/10.1186/1471-2407-8-161 Text en Copyright © 2008 Elluru et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Elluru, Sri Ramulu
van Huyen, Jean-Paul Duong
Delignat, Sandrine
Kazatchkine, Michel D
Friboulet, Alain
Kaveri, Srini V
Bayry, Jagadeesh
Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer
title Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer
title_full Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer
title_fullStr Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer
title_full_unstemmed Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer
title_short Induction of maturation and activation of human dendritic cells: A mechanism underlying the beneficial effect of Viscum album as complimentary therapy in cancer
title_sort induction of maturation and activation of human dendritic cells: a mechanism underlying the beneficial effect of viscum album as complimentary therapy in cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442603/
https://www.ncbi.nlm.nih.gov/pubmed/18533025
http://dx.doi.org/10.1186/1471-2407-8-161
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