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NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection
Immunity to the murine cytomegalovirus (MCMV) is critically dependent on the innate response for initial containment of viral replication, resolution of active infection, and proper induction of the adaptive phase of the anti-viral response. In contrast to NK cells, the Vα14 invariant natural killer...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442879/ https://www.ncbi.nlm.nih.gov/pubmed/18636102 http://dx.doi.org/10.1371/journal.ppat.1000106 |
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author | Wesley, Johnna D. Tessmer, Marlowe S. Chaukos, Deanna Brossay, Laurent |
author_facet | Wesley, Johnna D. Tessmer, Marlowe S. Chaukos, Deanna Brossay, Laurent |
author_sort | Wesley, Johnna D. |
collection | PubMed |
description | Immunity to the murine cytomegalovirus (MCMV) is critically dependent on the innate response for initial containment of viral replication, resolution of active infection, and proper induction of the adaptive phase of the anti-viral response. In contrast to NK cells, the Vα14 invariant natural killer T cell response to MCMV has not been examined. We found that Vα14i NK T cells become activated and produce significant levels of IFN-γ, but do not proliferate or produce IL-4 following MCMV infection. In vivo treatment with an anti-CD1d mAb and adoptive transfer of Vα14i NK T cells into MCMV-infected CD1d(−/−) mice demonstrate that CD1d is dispensable for Vα14i NK T cell activation. In contrast, both IFN-α/β and IL-12 are required for optimal activation. Vα14i NK T cell–derived IFN-γ is partially dependent on IFN-α/β but highly dependent on IL-12. Vα14i NK T cells contribute to the immune response to MCMV and amplify NK cell–derived IFN-γ. Importantly, mortality is increased in CD1d(−/−) mice in response to high dose MCMV infection when compared to heterozygote littermate controls. Collectively, these findings illustrate the plasticity of Vα14i NK T cells that act as effector T cells during bacterial infection, but have NK cell–like behavior during the innate immune response to MCMV infection. |
format | Text |
id | pubmed-2442879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-24428792008-07-18 NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection Wesley, Johnna D. Tessmer, Marlowe S. Chaukos, Deanna Brossay, Laurent PLoS Pathog Research Article Immunity to the murine cytomegalovirus (MCMV) is critically dependent on the innate response for initial containment of viral replication, resolution of active infection, and proper induction of the adaptive phase of the anti-viral response. In contrast to NK cells, the Vα14 invariant natural killer T cell response to MCMV has not been examined. We found that Vα14i NK T cells become activated and produce significant levels of IFN-γ, but do not proliferate or produce IL-4 following MCMV infection. In vivo treatment with an anti-CD1d mAb and adoptive transfer of Vα14i NK T cells into MCMV-infected CD1d(−/−) mice demonstrate that CD1d is dispensable for Vα14i NK T cell activation. In contrast, both IFN-α/β and IL-12 are required for optimal activation. Vα14i NK T cell–derived IFN-γ is partially dependent on IFN-α/β but highly dependent on IL-12. Vα14i NK T cells contribute to the immune response to MCMV and amplify NK cell–derived IFN-γ. Importantly, mortality is increased in CD1d(−/−) mice in response to high dose MCMV infection when compared to heterozygote littermate controls. Collectively, these findings illustrate the plasticity of Vα14i NK T cells that act as effector T cells during bacterial infection, but have NK cell–like behavior during the innate immune response to MCMV infection. Public Library of Science 2008-07-18 /pmc/articles/PMC2442879/ /pubmed/18636102 http://dx.doi.org/10.1371/journal.ppat.1000106 Text en Wesley et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wesley, Johnna D. Tessmer, Marlowe S. Chaukos, Deanna Brossay, Laurent NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection |
title | NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection |
title_full | NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection |
title_fullStr | NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection |
title_full_unstemmed | NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection |
title_short | NK Cell–Like Behavior of Vα14i NK T Cells during MCMV Infection |
title_sort | nk cell–like behavior of vα14i nk t cells during mcmv infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442879/ https://www.ncbi.nlm.nih.gov/pubmed/18636102 http://dx.doi.org/10.1371/journal.ppat.1000106 |
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