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Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures?
The occurrence of biliary stricutures in allografts following liver transplantation correlates with the duration of preservation time. The correlation between preservation time and biliary strictures suggests that anoxic or reperfusion injury of the bile duct epithelium causes stricture formation. H...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1996
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2443073/ https://www.ncbi.nlm.nih.gov/pubmed/8871253 http://dx.doi.org/10.1155/1996/48793 |
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author | Kahn, D. |
author_facet | Kahn, D. |
author_sort | Kahn, D. |
collection | PubMed |
description | The occurrence of biliary stricutures in allografts following liver transplantation correlates with the duration of preservation time. The correlation between preservation time and biliary strictures suggests that anoxic or reperfusion injury of the bile duct epithelium causes stricture formation. However, the relative susceptibility of bile duct cells to anoxic or reoxygenation injury is unknown. Our aims were to determine the vulnerability of rat liver bile duct cells to anoxic and reoxygenation injury and to compare the results with hepatocytes. During anoxia, bile duct epithelial cells were significantly more resistant to cell killing than hepatocytes. Rates of cellular proteolysis were also 2.5-fold lower in bile duct cells than in hepatocytes during anoxia. In contrast to anoxia, reoxygenation of anoxic cells increased cell killing of bile duct cells but improved viability of hepatocytes. The rate of toxic oxygen species formation by bile duct cells was 5-fold greater than in hepatocytes during reoxygenation. In addition, basal levels of glutathione are lower in bile duct cells than in hepatocytes. These data suggests that bile duct cells are more susceptible to reoxygenation injury than to anoxia. These studies support the hypothesis that reoxygenation injury during liver preservation leads to bile duct injury during liver transplantation. |
format | Text |
id | pubmed-2443073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1996 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-24430732008-07-08 Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? Kahn, D. HPB Surg Research Article The occurrence of biliary stricutures in allografts following liver transplantation correlates with the duration of preservation time. The correlation between preservation time and biliary strictures suggests that anoxic or reperfusion injury of the bile duct epithelium causes stricture formation. However, the relative susceptibility of bile duct cells to anoxic or reoxygenation injury is unknown. Our aims were to determine the vulnerability of rat liver bile duct cells to anoxic and reoxygenation injury and to compare the results with hepatocytes. During anoxia, bile duct epithelial cells were significantly more resistant to cell killing than hepatocytes. Rates of cellular proteolysis were also 2.5-fold lower in bile duct cells than in hepatocytes during anoxia. In contrast to anoxia, reoxygenation of anoxic cells increased cell killing of bile duct cells but improved viability of hepatocytes. The rate of toxic oxygen species formation by bile duct cells was 5-fold greater than in hepatocytes during reoxygenation. In addition, basal levels of glutathione are lower in bile duct cells than in hepatocytes. These data suggests that bile duct cells are more susceptible to reoxygenation injury than to anoxia. These studies support the hypothesis that reoxygenation injury during liver preservation leads to bile duct injury during liver transplantation. Hindawi Publishing Corporation 1996 /pmc/articles/PMC2443073/ /pubmed/8871253 http://dx.doi.org/10.1155/1996/48793 Text en Copyright © 1996 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kahn, D. Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? |
title | Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? |
title_full | Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? |
title_fullStr | Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? |
title_full_unstemmed | Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? |
title_short | Liver Transplantation: Does Prolonged Storage Promote Non-Anastomatic Biliary Structures? |
title_sort | liver transplantation: does prolonged storage promote non-anastomatic biliary structures? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2443073/ https://www.ncbi.nlm.nih.gov/pubmed/8871253 http://dx.doi.org/10.1155/1996/48793 |
work_keys_str_mv | AT kahnd livertransplantationdoesprolongedstoragepromotenonanastomaticbiliarystructures |