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Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data

The global analysis of proteins is now feasible due to improvements in techniques such as two-dimensional gel electrophoresis (2-DE), mass spectrometry, yeast two-hybrid systems and the development of bioinformatics applications. The experiments form the basis of proteomics, and present significant...

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Detalles Bibliográficos
Autores principales: Jones, Andrew, Wastling, Jonathan, Hunt, Ela
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447283/
https://www.ncbi.nlm.nih.gov/pubmed/18628860
http://dx.doi.org/10.1002/cfg.323
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author Jones, Andrew
Wastling, Jonathan
Hunt, Ela
author_facet Jones, Andrew
Wastling, Jonathan
Hunt, Ela
author_sort Jones, Andrew
collection PubMed
description The global analysis of proteins is now feasible due to improvements in techniques such as two-dimensional gel electrophoresis (2-DE), mass spectrometry, yeast two-hybrid systems and the development of bioinformatics applications. The experiments form the basis of proteomics, and present significant challenges in data analysis, storage and querying. We argue that a standard format for proteome data is required to enable the storage, exchange and subsequent re-analysis of large datasets. We describe the criteria that must be met for the development of a standard for proteomics. We have developed a model to represent data from 2-DE experiments, including difference gel electrophoresis along with image analysis and statistical analysis across multiple gels. This part of proteomics analysis is not represented in current proposals for proteomics standards. We are working with the Proteomics Standards Initiative to develop a model encompassing biological sample origin, experimental protocols, a number of separation techniques and mass spectrometry. The standard format will facilitate the development of central repositories of data, enabling results to be verified or re-analysed, and the correlation of results produced by different research groups using a variety of laboratory techniques.
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spelling pubmed-24472832008-07-14 Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data Jones, Andrew Wastling, Jonathan Hunt, Ela Comp Funct Genomics Research Article The global analysis of proteins is now feasible due to improvements in techniques such as two-dimensional gel electrophoresis (2-DE), mass spectrometry, yeast two-hybrid systems and the development of bioinformatics applications. The experiments form the basis of proteomics, and present significant challenges in data analysis, storage and querying. We argue that a standard format for proteome data is required to enable the storage, exchange and subsequent re-analysis of large datasets. We describe the criteria that must be met for the development of a standard for proteomics. We have developed a model to represent data from 2-DE experiments, including difference gel electrophoresis along with image analysis and statistical analysis across multiple gels. This part of proteomics analysis is not represented in current proposals for proteomics standards. We are working with the Proteomics Standards Initiative to develop a model encompassing biological sample origin, experimental protocols, a number of separation techniques and mass spectrometry. The standard format will facilitate the development of central repositories of data, enabling results to be verified or re-analysed, and the correlation of results produced by different research groups using a variety of laboratory techniques. Hindawi Publishing Corporation 2003-10 /pmc/articles/PMC2447283/ /pubmed/18628860 http://dx.doi.org/10.1002/cfg.323 Text en Copyright © 2003 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jones, Andrew
Wastling, Jonathan
Hunt, Ela
Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data
title Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data
title_full Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data
title_fullStr Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data
title_full_unstemmed Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data
title_short Proposal for a Standard Representation of Two-Dimensional Gel Electrophoresis Data
title_sort proposal for a standard representation of two-dimensional gel electrophoresis data
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447283/
https://www.ncbi.nlm.nih.gov/pubmed/18628860
http://dx.doi.org/10.1002/cfg.323
work_keys_str_mv AT jonesandrew proposalforastandardrepresentationoftwodimensionalgelelectrophoresisdata
AT wastlingjonathan proposalforastandardrepresentationoftwodimensionalgelelectrophoresisdata
AT huntela proposalforastandardrepresentationoftwodimensionalgelelectrophoresisdata