Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era

Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and pre...

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Autores principales: Landegren, Ulf, Dahl, Fredrik, Nilsson, Mats, Fredriksson, Simon, Banér, Johan, Gullberg, Mats, Jarvius, Jonas, Gustafsdottir, Sigrun, Söderberg, Ola, Ericsson, Olle, Stenberg, Johan, Schallmeiner, Edith
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2003
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447294/
https://www.ncbi.nlm.nih.gov/pubmed/18629012
http://dx.doi.org/10.1002/cfg.326
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author Landegren, Ulf
Dahl, Fredrik
Nilsson, Mats
Fredriksson, Simon
Banér, Johan
Gullberg, Mats
Jarvius, Jonas
Gustafsdottir, Sigrun
Söderberg, Ola
Ericsson, Olle
Stenberg, Johan
Schallmeiner, Edith
author_facet Landegren, Ulf
Dahl, Fredrik
Nilsson, Mats
Fredriksson, Simon
Banér, Johan
Gullberg, Mats
Jarvius, Jonas
Gustafsdottir, Sigrun
Söderberg, Ola
Ericsson, Olle
Stenberg, Johan
Schallmeiner, Edith
author_sort Landegren, Ulf
collection PubMed
description Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and present the approach our group has taken, involving the reformatting of analogue biological information to digital reporter segments of genetic information via a series of DNA ligation assays. The assays enable extensive, coordinated analyses of the numbers and locations of genes, transcripts and protein.
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spelling pubmed-24472942008-07-14 Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era Landegren, Ulf Dahl, Fredrik Nilsson, Mats Fredriksson, Simon Banér, Johan Gullberg, Mats Jarvius, Jonas Gustafsdottir, Sigrun Söderberg, Ola Ericsson, Olle Stenberg, Johan Schallmeiner, Edith Comp Funct Genomics Research Article Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and present the approach our group has taken, involving the reformatting of analogue biological information to digital reporter segments of genetic information via a series of DNA ligation assays. The assays enable extensive, coordinated analyses of the numbers and locations of genes, transcripts and protein. Hindawi Publishing Corporation 2003-10 /pmc/articles/PMC2447294/ /pubmed/18629012 http://dx.doi.org/10.1002/cfg.326 Text en Copyright © 2003 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Landegren, Ulf
Dahl, Fredrik
Nilsson, Mats
Fredriksson, Simon
Banér, Johan
Gullberg, Mats
Jarvius, Jonas
Gustafsdottir, Sigrun
Söderberg, Ola
Ericsson, Olle
Stenberg, Johan
Schallmeiner, Edith
Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
title Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
title_full Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
title_fullStr Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
title_full_unstemmed Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
title_short Padlock and Proximity Probes for in Situ and Array-Based Analyses: Tools for the Post-Genomic Era
title_sort padlock and proximity probes for in situ and array-based analyses: tools for the post-genomic era
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447294/
https://www.ncbi.nlm.nih.gov/pubmed/18629012
http://dx.doi.org/10.1002/cfg.326
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