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Myosin-10 and actin filaments are essential for mitotic spindle function

Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division. Although the roles of microtubules and microtubule-based motors in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin–based motors (myosins) are re...

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Detalles Bibliográficos
Autores principales: Woolner, Sarah, O'Brien, Lori L., Wiese, Christiane, Bement, William M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447898/
https://www.ncbi.nlm.nih.gov/pubmed/18606852
http://dx.doi.org/10.1083/jcb.200804062
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author Woolner, Sarah
O'Brien, Lori L.
Wiese, Christiane
Bement, William M.
author_facet Woolner, Sarah
O'Brien, Lori L.
Wiese, Christiane
Bement, William M.
author_sort Woolner, Sarah
collection PubMed
description Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division. Although the roles of microtubules and microtubule-based motors in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin–based motors (myosins) are required components of mitotic spindles has long been controversial. Based on the demonstration that myosin-10 (Myo10) is important for assembly of meiotic spindles, we assessed the role of this unconventional myosin, as well as F-actin, in mitotic spindles. We find that Myo10 localizes to mitotic spindle poles and is essential for proper spindle anchoring, normal spindle length, spindle pole integrity, and progression through metaphase. Furthermore, we show that F-actin localizes to mitotic spindles in dynamic cables that surround the spindle and extend between the spindle and the cortex. Remarkably, although proper anchoring depends on both F-actin and Myo10, the requirement for Myo10 in spindle pole integrity is F-actin independent, whereas F-actin and Myo10 actually play antagonistic roles in maintenance of spindle length.
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spelling pubmed-24478982009-01-14 Myosin-10 and actin filaments are essential for mitotic spindle function Woolner, Sarah O'Brien, Lori L. Wiese, Christiane Bement, William M. J Cell Biol Research Articles Mitotic spindles are microtubule-based structures responsible for chromosome partitioning during cell division. Although the roles of microtubules and microtubule-based motors in mitotic spindles are well established, whether or not actin filaments (F-actin) and F-actin–based motors (myosins) are required components of mitotic spindles has long been controversial. Based on the demonstration that myosin-10 (Myo10) is important for assembly of meiotic spindles, we assessed the role of this unconventional myosin, as well as F-actin, in mitotic spindles. We find that Myo10 localizes to mitotic spindle poles and is essential for proper spindle anchoring, normal spindle length, spindle pole integrity, and progression through metaphase. Furthermore, we show that F-actin localizes to mitotic spindles in dynamic cables that surround the spindle and extend between the spindle and the cortex. Remarkably, although proper anchoring depends on both F-actin and Myo10, the requirement for Myo10 in spindle pole integrity is F-actin independent, whereas F-actin and Myo10 actually play antagonistic roles in maintenance of spindle length. The Rockefeller University Press 2008-07-14 /pmc/articles/PMC2447898/ /pubmed/18606852 http://dx.doi.org/10.1083/jcb.200804062 Text en © 2008 Woolner et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Woolner, Sarah
O'Brien, Lori L.
Wiese, Christiane
Bement, William M.
Myosin-10 and actin filaments are essential for mitotic spindle function
title Myosin-10 and actin filaments are essential for mitotic spindle function
title_full Myosin-10 and actin filaments are essential for mitotic spindle function
title_fullStr Myosin-10 and actin filaments are essential for mitotic spindle function
title_full_unstemmed Myosin-10 and actin filaments are essential for mitotic spindle function
title_short Myosin-10 and actin filaments are essential for mitotic spindle function
title_sort myosin-10 and actin filaments are essential for mitotic spindle function
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447898/
https://www.ncbi.nlm.nih.gov/pubmed/18606852
http://dx.doi.org/10.1083/jcb.200804062
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