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Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells

Deadenylation is the major step triggering mammalian mRNA decay. One consequence of deadenylation is the formation of nontranslatable messenger RNA (mRNA) protein complexes (messenger ribonucleoproteins [mRNPs]). Nontranslatable mRNPs may accumulate in P-bodies, which contain factors involved in tra...

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Autores principales: Zheng, Dinghai, Ezzeddine, Nader, Chen, Chyi-Ying A., Zhu, Wenmiao, He, Xiangwei, Shyu, Ann-Bin
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447901/
https://www.ncbi.nlm.nih.gov/pubmed/18625844
http://dx.doi.org/10.1083/jcb.200801196
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author Zheng, Dinghai
Ezzeddine, Nader
Chen, Chyi-Ying A.
Zhu, Wenmiao
He, Xiangwei
Shyu, Ann-Bin
author_facet Zheng, Dinghai
Ezzeddine, Nader
Chen, Chyi-Ying A.
Zhu, Wenmiao
He, Xiangwei
Shyu, Ann-Bin
author_sort Zheng, Dinghai
collection PubMed
description Deadenylation is the major step triggering mammalian mRNA decay. One consequence of deadenylation is the formation of nontranslatable messenger RNA (mRNA) protein complexes (messenger ribonucleoproteins [mRNPs]). Nontranslatable mRNPs may accumulate in P-bodies, which contain factors involved in translation repression, decapping, and 5′-to-3′ degradation. We demonstrate that deadenylation is required for mammalian P-body formation and mRNA decay. We identify Pan2, Pan3, and Caf1 deadenylases as new P-body components and show that Pan3 helps recruit Pan2, Ccr4, and Caf1 to P-bodies. Pan3 knockdown causes a reduction of P-bodies and has differential effects on mRNA decay. Knocking down Caf1 or overexpressing a Caf1 catalytically inactive mutant impairs deadenylation and mRNA decay. P-bodies are not detected when deadenylation is blocked and are restored when the blockage is released. When deadenylation is impaired, P-body formation is not restorable, even when mRNAs exit the translating pool. These results support a dynamic interplay among deadenylation, mRNP remodeling, and P-body formation in selective decay of mammalian mRNA.
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spelling pubmed-24479012009-01-14 Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells Zheng, Dinghai Ezzeddine, Nader Chen, Chyi-Ying A. Zhu, Wenmiao He, Xiangwei Shyu, Ann-Bin J Cell Biol Research Articles Deadenylation is the major step triggering mammalian mRNA decay. One consequence of deadenylation is the formation of nontranslatable messenger RNA (mRNA) protein complexes (messenger ribonucleoproteins [mRNPs]). Nontranslatable mRNPs may accumulate in P-bodies, which contain factors involved in translation repression, decapping, and 5′-to-3′ degradation. We demonstrate that deadenylation is required for mammalian P-body formation and mRNA decay. We identify Pan2, Pan3, and Caf1 deadenylases as new P-body components and show that Pan3 helps recruit Pan2, Ccr4, and Caf1 to P-bodies. Pan3 knockdown causes a reduction of P-bodies and has differential effects on mRNA decay. Knocking down Caf1 or overexpressing a Caf1 catalytically inactive mutant impairs deadenylation and mRNA decay. P-bodies are not detected when deadenylation is blocked and are restored when the blockage is released. When deadenylation is impaired, P-body formation is not restorable, even when mRNAs exit the translating pool. These results support a dynamic interplay among deadenylation, mRNP remodeling, and P-body formation in selective decay of mammalian mRNA. The Rockefeller University Press 2008-07-14 /pmc/articles/PMC2447901/ /pubmed/18625844 http://dx.doi.org/10.1083/jcb.200801196 Text en © 2008 Zheng et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Zheng, Dinghai
Ezzeddine, Nader
Chen, Chyi-Ying A.
Zhu, Wenmiao
He, Xiangwei
Shyu, Ann-Bin
Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells
title Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells
title_full Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells
title_fullStr Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells
title_full_unstemmed Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells
title_short Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells
title_sort deadenylation is prerequisite for p-body formation and mrna decay in mammalian cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2447901/
https://www.ncbi.nlm.nih.gov/pubmed/18625844
http://dx.doi.org/10.1083/jcb.200801196
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