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Global cDNA Amplification Combined with Real-Time RT–PCR: Accurate Quantification of Multiple Human Potassium Channel Genes at the Single Cell Level

We have developed a sensitive quantitative RT–PCR procedure suitable for the analysis of small samples, including single cells, and have used it to measure levels of potassium channel mRNAs in a panel of human tissues and small numbers of cells grown in culture. The method involves an initial global...

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Detalles Bibliográficos
Autores principales:	Al-Taher, A., Bashein, A., Nolan, T., Hollingsworth, M., Brady, G.
Formato:	Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2000
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2448369/ https://www.ncbi.nlm.nih.gov/pubmed/11025530 http://dx.doi.org/10.1002/1097-0061(20000930)17:3<201::AID-YEA30>3.0.CO;2-R

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