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Haemophilus Influenzae Microarrays: Virulence and Vaccines
In 1995 the genome sequence of the Haemophilus influenzae KW20 (Rd) strain was published, the first available for a free-living organism. The genome has been invaluable in global strategies to identify certain virulence-related genes, e.g. those involved in LPS synthesis, and also essential genes, b...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2448429/ https://www.ncbi.nlm.nih.gov/pubmed/18629266 http://dx.doi.org/10.1002/cfg.194 |
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author | Ali, Tahir R. Kroll, J. Simon Langford, Paul R. |
author_facet | Ali, Tahir R. Kroll, J. Simon Langford, Paul R. |
author_sort | Ali, Tahir R. |
collection | PubMed |
description | In 1995 the genome sequence of the Haemophilus influenzae KW20 (Rd) strain was published, the first available for a free-living organism. The genome has been invaluable in global strategies to identify certain virulence-related genes, e.g. those involved in LPS synthesis, and also essential genes, but there is a paucity of wholegenome transcriptome studies. We have now constructed a whole-genome array consisting of genes from Rd, additional genes identified in other strains of H. influenzae and controls (from eukaryotic sources and other bacteria). We intend to use this array in studies aimed at understanding the bacterium’s basic metabolism and its response to changing environments; deciphering global regulatory networks (by comparison of wild-type and mutant strains); and identifying genes expressed in vivo. The use of H. influenzae DNA arrays combined with proteomic approaches will enhance our understanding of the metabolism and virulence of the organism. Additionally, the genome sequence of a non-typable H. influenzae strain is in progress. The sequence from this isolate will be invaluable not only in identifying potential novel antibiotic targets and putative vaccine candidates but also in the design of a microarray for genome-typing purposes. |
format | Text |
id | pubmed-2448429 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-24484292008-07-14 Haemophilus Influenzae Microarrays: Virulence and Vaccines Ali, Tahir R. Kroll, J. Simon Langford, Paul R. Comp Funct Genomics Research Article In 1995 the genome sequence of the Haemophilus influenzae KW20 (Rd) strain was published, the first available for a free-living organism. The genome has been invaluable in global strategies to identify certain virulence-related genes, e.g. those involved in LPS synthesis, and also essential genes, but there is a paucity of wholegenome transcriptome studies. We have now constructed a whole-genome array consisting of genes from Rd, additional genes identified in other strains of H. influenzae and controls (from eukaryotic sources and other bacteria). We intend to use this array in studies aimed at understanding the bacterium’s basic metabolism and its response to changing environments; deciphering global regulatory networks (by comparison of wild-type and mutant strains); and identifying genes expressed in vivo. The use of H. influenzae DNA arrays combined with proteomic approaches will enhance our understanding of the metabolism and virulence of the organism. Additionally, the genome sequence of a non-typable H. influenzae strain is in progress. The sequence from this isolate will be invaluable not only in identifying potential novel antibiotic targets and putative vaccine candidates but also in the design of a microarray for genome-typing purposes. Hindawi Publishing Corporation 2002-08 /pmc/articles/PMC2448429/ /pubmed/18629266 http://dx.doi.org/10.1002/cfg.194 Text en Copyright © 2002 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ali, Tahir R. Kroll, J. Simon Langford, Paul R. Haemophilus Influenzae Microarrays: Virulence and Vaccines |
title | Haemophilus Influenzae Microarrays: Virulence and Vaccines |
title_full | Haemophilus Influenzae Microarrays: Virulence and Vaccines |
title_fullStr | Haemophilus Influenzae Microarrays: Virulence and Vaccines |
title_full_unstemmed | Haemophilus Influenzae Microarrays: Virulence and Vaccines |
title_short | Haemophilus Influenzae Microarrays: Virulence and Vaccines |
title_sort | haemophilus influenzae microarrays: virulence and vaccines |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2448429/ https://www.ncbi.nlm.nih.gov/pubmed/18629266 http://dx.doi.org/10.1002/cfg.194 |
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