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The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae
Streptococcus pneumoniae is an important human pathogen associated with pneumonia, septicaemia, meningitis and otitis media. It is estimated to result in over 3 million child deaths worldwide every year and an even greater number of deaths among the elderly. Prior to the complete sequencing of the g...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2448440/ https://www.ncbi.nlm.nih.gov/pubmed/18629276 http://dx.doi.org/10.1002/cfg.190 |
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author | McCluskey, Jackie Dowson, Christopher G. Mitchell, Timothy J. |
author_facet | McCluskey, Jackie Dowson, Christopher G. Mitchell, Timothy J. |
author_sort | McCluskey, Jackie |
collection | PubMed |
description | Streptococcus pneumoniae is an important human pathogen associated with pneumonia, septicaemia, meningitis and otitis media. It is estimated to result in over 3 million child deaths worldwide every year and an even greater number of deaths among the elderly. Prior to the complete sequencing of the genomes of S. pneumoniae TIGR4 (serotype 4) and S. pneumoniae R6 (serotype 2), we designed a custom miniarray consisting of 497 pneumococcal genes. The overall objectives of our microarray investigations were, first, to assess the genetic diversity between different S. pneumoniae serotypes, clinical isolates and also different Streptococcus species; second, we aimed to use microarray technology to examine the mechanisms by which environmental factors influence pneumococcal gene expression, and ultimately to further the understanding of how these changes in gene expression are achieved and how they may alter the virulence of the organism. |
format | Text |
id | pubmed-2448440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-24484402008-07-14 The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae McCluskey, Jackie Dowson, Christopher G. Mitchell, Timothy J. Comp Funct Genomics Research Article Streptococcus pneumoniae is an important human pathogen associated with pneumonia, septicaemia, meningitis and otitis media. It is estimated to result in over 3 million child deaths worldwide every year and an even greater number of deaths among the elderly. Prior to the complete sequencing of the genomes of S. pneumoniae TIGR4 (serotype 4) and S. pneumoniae R6 (serotype 2), we designed a custom miniarray consisting of 497 pneumococcal genes. The overall objectives of our microarray investigations were, first, to assess the genetic diversity between different S. pneumoniae serotypes, clinical isolates and also different Streptococcus species; second, we aimed to use microarray technology to examine the mechanisms by which environmental factors influence pneumococcal gene expression, and ultimately to further the understanding of how these changes in gene expression are achieved and how they may alter the virulence of the organism. Hindawi Publishing Corporation 2002-08 /pmc/articles/PMC2448440/ /pubmed/18629276 http://dx.doi.org/10.1002/cfg.190 Text en Copyright © 2002 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article McCluskey, Jackie Dowson, Christopher G. Mitchell, Timothy J. The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae |
title | The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae |
title_full | The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae |
title_fullStr | The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae |
title_full_unstemmed | The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae |
title_short | The Use of Microarray Technology for the Analysis of Streptococcus Pneumoniae |
title_sort | use of microarray technology for the analysis of streptococcus pneumoniae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2448440/ https://www.ncbi.nlm.nih.gov/pubmed/18629276 http://dx.doi.org/10.1002/cfg.190 |
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