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Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains

OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypo...

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Autores principales: Berglund, Eric D., Li, Candice Y., Poffenberger, Greg, Ayala, Julio E., Fueger, Patrick T., Willis, Shannon E., Jewell, Marybeth M., Powers, Alvin C., Wasserman, David H.
Formato: Texto
Lenguaje:English
Publicado: American Diabetes Association 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2453626/
https://www.ncbi.nlm.nih.gov/pubmed/18398139
http://dx.doi.org/10.2337/db07-1615
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author Berglund, Eric D.
Li, Candice Y.
Poffenberger, Greg
Ayala, Julio E.
Fueger, Patrick T.
Willis, Shannon E.
Jewell, Marybeth M.
Powers, Alvin C.
Wasserman, David H.
author_facet Berglund, Eric D.
Li, Candice Y.
Poffenberger, Greg
Ayala, Julio E.
Fueger, Patrick T.
Willis, Shannon E.
Jewell, Marybeth M.
Powers, Alvin C.
Wasserman, David H.
author_sort Berglund, Eric D.
collection PubMed
description OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypoglycemic counter-regulatory responsiveness. Hyperglycemic clamps (∼15 mmol/l) were done to assess insulin secretion and compared with results in perifused islets. RESULTS—Insulin action and hypoglycemic counter-regulatory and insulin secretory phenotypes varied considerably in four inbred mouse strains. In vivo insulin secretion was greatest in 129X1/Sv mice, but the counter-regulatory response to hypoglycemia was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin secretion, relative hepatic insulin resistance, and the highest counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was lowest among the strains, and islets isolated had the greatest glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were intermediate relative to other strains. Insulin secretion by C57BL/6 mice was similar to that in other strains in contrast to the blunted glucose-stimulated insulin secretion from isolated islets. CONCLUSIONS—Strain-dependent differences exist in four inbred mouse strains frequently used for genetic manipulation and study of glucose metabolism. These results are important for selecting inbred mice to study glucose metabolism and for interpreting and designing experiments.
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spelling pubmed-24536262009-07-01 Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains Berglund, Eric D. Li, Candice Y. Poffenberger, Greg Ayala, Julio E. Fueger, Patrick T. Willis, Shannon E. Jewell, Marybeth M. Powers, Alvin C. Wasserman, David H. Diabetes New Methodologies and Databases OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypoglycemic counter-regulatory responsiveness. Hyperglycemic clamps (∼15 mmol/l) were done to assess insulin secretion and compared with results in perifused islets. RESULTS—Insulin action and hypoglycemic counter-regulatory and insulin secretory phenotypes varied considerably in four inbred mouse strains. In vivo insulin secretion was greatest in 129X1/Sv mice, but the counter-regulatory response to hypoglycemia was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin secretion, relative hepatic insulin resistance, and the highest counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was lowest among the strains, and islets isolated had the greatest glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were intermediate relative to other strains. Insulin secretion by C57BL/6 mice was similar to that in other strains in contrast to the blunted glucose-stimulated insulin secretion from isolated islets. CONCLUSIONS—Strain-dependent differences exist in four inbred mouse strains frequently used for genetic manipulation and study of glucose metabolism. These results are important for selecting inbred mice to study glucose metabolism and for interpreting and designing experiments. American Diabetes Association 2008-07 /pmc/articles/PMC2453626/ /pubmed/18398139 http://dx.doi.org/10.2337/db07-1615 Text en Copyright © 2008, American Diabetes Association https://creativecommons.org/licenses/by-nc-nd/3.0/Readers may use this article as long as the work is properly cited, the use is educational and not for profit,and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.
spellingShingle New Methodologies and Databases
Berglund, Eric D.
Li, Candice Y.
Poffenberger, Greg
Ayala, Julio E.
Fueger, Patrick T.
Willis, Shannon E.
Jewell, Marybeth M.
Powers, Alvin C.
Wasserman, David H.
Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
title Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
title_full Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
title_fullStr Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
title_full_unstemmed Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
title_short Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
title_sort glucose metabolism in vivo in four commonly used inbred mouse strains
topic New Methodologies and Databases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2453626/
https://www.ncbi.nlm.nih.gov/pubmed/18398139
http://dx.doi.org/10.2337/db07-1615
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