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Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains
OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypo...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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American Diabetes Association
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2453626/ https://www.ncbi.nlm.nih.gov/pubmed/18398139 http://dx.doi.org/10.2337/db07-1615 |
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author | Berglund, Eric D. Li, Candice Y. Poffenberger, Greg Ayala, Julio E. Fueger, Patrick T. Willis, Shannon E. Jewell, Marybeth M. Powers, Alvin C. Wasserman, David H. |
author_facet | Berglund, Eric D. Li, Candice Y. Poffenberger, Greg Ayala, Julio E. Fueger, Patrick T. Willis, Shannon E. Jewell, Marybeth M. Powers, Alvin C. Wasserman, David H. |
author_sort | Berglund, Eric D. |
collection | PubMed |
description | OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypoglycemic counter-regulatory responsiveness. Hyperglycemic clamps (∼15 mmol/l) were done to assess insulin secretion and compared with results in perifused islets. RESULTS—Insulin action and hypoglycemic counter-regulatory and insulin secretory phenotypes varied considerably in four inbred mouse strains. In vivo insulin secretion was greatest in 129X1/Sv mice, but the counter-regulatory response to hypoglycemia was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin secretion, relative hepatic insulin resistance, and the highest counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was lowest among the strains, and islets isolated had the greatest glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were intermediate relative to other strains. Insulin secretion by C57BL/6 mice was similar to that in other strains in contrast to the blunted glucose-stimulated insulin secretion from isolated islets. CONCLUSIONS—Strain-dependent differences exist in four inbred mouse strains frequently used for genetic manipulation and study of glucose metabolism. These results are important for selecting inbred mice to study glucose metabolism and for interpreting and designing experiments. |
format | Text |
id | pubmed-2453626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-24536262009-07-01 Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains Berglund, Eric D. Li, Candice Y. Poffenberger, Greg Ayala, Julio E. Fueger, Patrick T. Willis, Shannon E. Jewell, Marybeth M. Powers, Alvin C. Wasserman, David H. Diabetes New Methodologies and Databases OBJECTIVE—To characterize differences in whole-body glucose metabolism between commonly used inbred mouse strains. RESEARCH DESIGN AND METHODS—Hyperinsulinemic-euglycemic (∼8.5 mmol/l) and -hypoglycemic (∼3.0 mmol/l) clamps were done in catheterized, 5-h-fasted mice to assess insulin action and hypoglycemic counter-regulatory responsiveness. Hyperglycemic clamps (∼15 mmol/l) were done to assess insulin secretion and compared with results in perifused islets. RESULTS—Insulin action and hypoglycemic counter-regulatory and insulin secretory phenotypes varied considerably in four inbred mouse strains. In vivo insulin secretion was greatest in 129X1/Sv mice, but the counter-regulatory response to hypoglycemia was blunted. FVB/N mice in vivo showed no increase in glucose-stimulated insulin secretion, relative hepatic insulin resistance, and the highest counter-regulatory response to hypoglycemia. In DBA/2 mice, insulin action was lowest among the strains, and islets isolated had the greatest glucose-stimulated insulin secretion in vitro. In C57BL/6 mice, in vivo physiological responses to hyperinsulinemia at euglycemia and hypoglycemia were intermediate relative to other strains. Insulin secretion by C57BL/6 mice was similar to that in other strains in contrast to the blunted glucose-stimulated insulin secretion from isolated islets. CONCLUSIONS—Strain-dependent differences exist in four inbred mouse strains frequently used for genetic manipulation and study of glucose metabolism. These results are important for selecting inbred mice to study glucose metabolism and for interpreting and designing experiments. American Diabetes Association 2008-07 /pmc/articles/PMC2453626/ /pubmed/18398139 http://dx.doi.org/10.2337/db07-1615 Text en Copyright © 2008, American Diabetes Association https://creativecommons.org/licenses/by-nc-nd/3.0/Readers may use this article as long as the work is properly cited, the use is educational and not for profit,and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details. |
spellingShingle | New Methodologies and Databases Berglund, Eric D. Li, Candice Y. Poffenberger, Greg Ayala, Julio E. Fueger, Patrick T. Willis, Shannon E. Jewell, Marybeth M. Powers, Alvin C. Wasserman, David H. Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains |
title | Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains |
title_full | Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains |
title_fullStr | Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains |
title_full_unstemmed | Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains |
title_short | Glucose Metabolism In Vivo in Four Commonly Used Inbred Mouse Strains |
title_sort | glucose metabolism in vivo in four commonly used inbred mouse strains |
topic | New Methodologies and Databases |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2453626/ https://www.ncbi.nlm.nih.gov/pubmed/18398139 http://dx.doi.org/10.2337/db07-1615 |
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