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Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study
BACKGROUND: The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2459177/ https://www.ncbi.nlm.nih.gov/pubmed/18597674 http://dx.doi.org/10.1186/1477-7827-6-27 |
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author | Kralova, Alena Svetlikova, Marta Madar, Jindrich Ulcova-Gallova, Zdena Bukovsky, Antonin Peknicova, Jana |
author_facet | Kralova, Alena Svetlikova, Marta Madar, Jindrich Ulcova-Gallova, Zdena Bukovsky, Antonin Peknicova, Jana |
author_sort | Kralova, Alena |
collection | PubMed |
description | BACKGROUND: The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction. METHODS: In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies. RESULTS: Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p < 0.001) when compared to placentae from abnormal ones (gestational diabetes, pregnancy induced hypertension, drug abuse). CONCLUSION: These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction. |
format | Text |
id | pubmed-2459177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-24591772008-07-12 Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study Kralova, Alena Svetlikova, Marta Madar, Jindrich Ulcova-Gallova, Zdena Bukovsky, Antonin Peknicova, Jana Reprod Biol Endocrinol Research BACKGROUND: The placenta is an important site for iron metabolism in humans. It transfers iron from the mother to the fetus. One of the major iron transport proteins is transferrin, which is a blood plasma protein crucial for iron uptake. Its localization and expression may be one of the markers to distinguish placental dysfunction. METHODS: In the experimental study we used antibody preparation, mass spectrometric analysis, biochemical and immunocytochemical methods for characterization of transferrin expression on the human choriocarcinoma cell line JAR (JAR cells), placental lysates, and cryostat sections. Newly designed monoclonal antibody TRO-tf-01 to human transferrin was applied on human placentae from normal (n = 3) and abnormal (n = 9) pregnancies. RESULTS: Variations of transferrin expression were detected in villous syncytiotrophoblast, which is in direct contact with maternal blood. In placentae from normal pregnancies, the expression of transferrin in the syncytium was significantly lower (p < 0.001) when compared to placentae from abnormal ones (gestational diabetes, pregnancy induced hypertension, drug abuse). CONCLUSION: These observations suggest that in the case of abnormal pregnancies, the fetus may require higher levels of transferrin in order to prevent iron depletion due to the stress from the placental dysfunction. BioMed Central 2008-07-02 /pmc/articles/PMC2459177/ /pubmed/18597674 http://dx.doi.org/10.1186/1477-7827-6-27 Text en Copyright © 2008 Kralova et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Kralova, Alena Svetlikova, Marta Madar, Jindrich Ulcova-Gallova, Zdena Bukovsky, Antonin Peknicova, Jana Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
title | Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
title_full | Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
title_fullStr | Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
title_full_unstemmed | Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
title_short | Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
title_sort | differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2459177/ https://www.ncbi.nlm.nih.gov/pubmed/18597674 http://dx.doi.org/10.1186/1477-7827-6-27 |
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