A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry

Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is sufficient to assemble the entire exterior shell of the barrel...

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Autores principales: Kato, Koji, Tanaka, Hideaki, Sumizawa, Tomoyuki, Yoshimura, Masato, Yamashita, Eiki, Iwasaki, Kenji, Tsukihara, Tomitake
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2008
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2467523/
https://www.ncbi.nlm.nih.gov/pubmed/18453688
http://dx.doi.org/10.1107/S0907444908004277
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author Kato, Koji
Tanaka, Hideaki
Sumizawa, Tomoyuki
Yoshimura, Masato
Yamashita, Eiki
Iwasaki, Kenji
Tsukihara, Tomitake
author_facet Kato, Koji
Tanaka, Hideaki
Sumizawa, Tomoyuki
Yoshimura, Masato
Yamashita, Eiki
Iwasaki, Kenji
Tsukihara, Tomitake
author_sort Kato, Koji
collection PubMed
description Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is sufficient to assemble the entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, vault poly(ADP-ribose) polymerase (VPARP) and telomerase-associated protein 1 (TEP1), as well as a small vault RNA (vRNA), are also associated with vault. Here, the crystallization of vault particles is reported. The crystals belong to space group C2, with unit-cell parameters a = 708.0, b = 385.0, c = 602.9 Å, β = 124.8°. Rotational symmetry searches based on the R factor and correlation coefficient from noncrystallographic symmetry (NCS) averaging indicated that the particle has 39-fold dihedral symmetry.
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spelling pubmed-24675232009-03-05 A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry Kato, Koji Tanaka, Hideaki Sumizawa, Tomoyuki Yoshimura, Masato Yamashita, Eiki Iwasaki, Kenji Tsukihara, Tomitake Acta Crystallogr D Biol Crystallogr Research Papers Vault is a 12.9 MDa ribonucleoprotein particle with a barrel-like shape, two protruding caps and an invaginated waist structure that is highly conserved in a wide variety of eukaryotes. Multimerization of the major vault protein (MVP) is sufficient to assemble the entire exterior shell of the barrel-shaped vault particle. Multiple copies of two additional proteins, vault poly(ADP-ribose) polymerase (VPARP) and telomerase-associated protein 1 (TEP1), as well as a small vault RNA (vRNA), are also associated with vault. Here, the crystallization of vault particles is reported. The crystals belong to space group C2, with unit-cell parameters a = 708.0, b = 385.0, c = 602.9 Å, β = 124.8°. Rotational symmetry searches based on the R factor and correlation coefficient from noncrystallographic symmetry (NCS) averaging indicated that the particle has 39-fold dihedral symmetry. International Union of Crystallography 2008-05-01 2008-04-19 /pmc/articles/PMC2467523/ /pubmed/18453688 http://dx.doi.org/10.1107/S0907444908004277 Text en © International Union of Crystallography 2008 http://journals.iucr.org/services/termsofuse.html This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.
spellingShingle Research Papers
Kato, Koji
Tanaka, Hideaki
Sumizawa, Tomoyuki
Yoshimura, Masato
Yamashita, Eiki
Iwasaki, Kenji
Tsukihara, Tomitake
A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
title A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
title_full A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
title_fullStr A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
title_full_unstemmed A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
title_short A vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
title_sort vault ribonucleoprotein particle exhibiting 39-fold dihedral symmetry
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2467523/
https://www.ncbi.nlm.nih.gov/pubmed/18453688
http://dx.doi.org/10.1107/S0907444908004277
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