Cargando…
Regulation of hypoxia-inducible factor-1α by NF-κB
HIF (hypoxia-inducible factor) is the main transcription factor activated by low oxygen tensions. HIF-1α (and other α subunits) is tightly controlled mostly at the protein level, through the concerted action of a class of enzymes called PHDs (prolyl hydroxylases) 1, 2 and 3. Most of the knowledge of...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2474706/ https://www.ncbi.nlm.nih.gov/pubmed/18393939 http://dx.doi.org/10.1042/BJ20080476 |
_version_ | 1782157504894992384 |
---|---|
author | van Uden, Patrick Kenneth, Niall S. Rocha, Sonia |
author_facet | van Uden, Patrick Kenneth, Niall S. Rocha, Sonia |
author_sort | van Uden, Patrick |
collection | PubMed |
description | HIF (hypoxia-inducible factor) is the main transcription factor activated by low oxygen tensions. HIF-1α (and other α subunits) is tightly controlled mostly at the protein level, through the concerted action of a class of enzymes called PHDs (prolyl hydroxylases) 1, 2 and 3. Most of the knowledge of HIF derives from studies following hypoxic stress; however, HIF-1α stabilization is also found in non-hypoxic conditions through an unknown mechanism. In the present study, we demonstrate that NF-κB (nuclear factor κB) is a direct modulator of HIF-1α expression. The HIF-1α promoter is responsive to selective NF-κB subunits. siRNA (small interfering RNA) studies for individual NF-κB members revealed differential effects on HIF-1α mRNA levels, indicating that NF-κB can regulate basal HIF-1α expression. Finally, when endogenous NF-κB is induced by TNFα (tumour necrosis factor α) treatment, HIF-1α levels also change in an NF-κB-dependent manner. In conclusion, we find that NF-κB can regulate basal TNFα and, in certain circumstances, the hypoxia-induced HIF-1α. |
format | Text |
id | pubmed-2474706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-24747062008-10-07 Regulation of hypoxia-inducible factor-1α by NF-κB van Uden, Patrick Kenneth, Niall S. Rocha, Sonia Biochem J Research Article HIF (hypoxia-inducible factor) is the main transcription factor activated by low oxygen tensions. HIF-1α (and other α subunits) is tightly controlled mostly at the protein level, through the concerted action of a class of enzymes called PHDs (prolyl hydroxylases) 1, 2 and 3. Most of the knowledge of HIF derives from studies following hypoxic stress; however, HIF-1α stabilization is also found in non-hypoxic conditions through an unknown mechanism. In the present study, we demonstrate that NF-κB (nuclear factor κB) is a direct modulator of HIF-1α expression. The HIF-1α promoter is responsive to selective NF-κB subunits. siRNA (small interfering RNA) studies for individual NF-κB members revealed differential effects on HIF-1α mRNA levels, indicating that NF-κB can regulate basal HIF-1α expression. Finally, when endogenous NF-κB is induced by TNFα (tumour necrosis factor α) treatment, HIF-1α levels also change in an NF-κB-dependent manner. In conclusion, we find that NF-κB can regulate basal TNFα and, in certain circumstances, the hypoxia-induced HIF-1α. Portland Press Ltd. 2008-05-28 2008-06-15 /pmc/articles/PMC2474706/ /pubmed/18393939 http://dx.doi.org/10.1042/BJ20080476 Text en © 2008 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited http://creativecommons.org/licenses/by-nc/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article van Uden, Patrick Kenneth, Niall S. Rocha, Sonia Regulation of hypoxia-inducible factor-1α by NF-κB |
title | Regulation of hypoxia-inducible factor-1α by NF-κB |
title_full | Regulation of hypoxia-inducible factor-1α by NF-κB |
title_fullStr | Regulation of hypoxia-inducible factor-1α by NF-κB |
title_full_unstemmed | Regulation of hypoxia-inducible factor-1α by NF-κB |
title_short | Regulation of hypoxia-inducible factor-1α by NF-κB |
title_sort | regulation of hypoxia-inducible factor-1α by nf-κb |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2474706/ https://www.ncbi.nlm.nih.gov/pubmed/18393939 http://dx.doi.org/10.1042/BJ20080476 |
work_keys_str_mv | AT vanudenpatrick regulationofhypoxiainduciblefactor1abynfkb AT kennethnialls regulationofhypoxiainduciblefactor1abynfkb AT rochasonia regulationofhypoxiainduciblefactor1abynfkb |