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Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus

Aqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the...

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Autores principales: Martins, R. F., Ramos, M. F., Herfindal, L., Sousa, J. A., Skærven, K., Vasconcelos, V. M.
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2474953/
https://www.ncbi.nlm.nih.gov/pubmed/18648669
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author Martins, R. F.
Ramos, M. F.
Herfindal, L.
Sousa, J. A.
Skærven, K.
Vasconcelos, V. M.
author_facet Martins, R. F.
Ramos, M. F.
Herfindal, L.
Sousa, J. A.
Skærven, K.
Vasconcelos, V. M.
author_sort Martins, R. F.
collection PubMed
description Aqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the agar diffusion assay. Cytotoxic activity was measured by apoptotic cell death scored by cell surface evaluation and nuclear morphology. A high percentage of apoptotic cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Slight apoptotic effects were observed in primary rat hepatocytes when exposed to aqueous cyanobacterial extracts. Nine cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria, Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. No inhibitory effects were found against the fungus Candida albicans and Gram-negative bacteria. Marine Synechocystis and Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Gram-positive bacteria. The different activity in different extracts suggests different compounds with different polarities.
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spelling pubmed-24749532008-07-22 Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus Martins, R. F. Ramos, M. F. Herfindal, L. Sousa, J. A. Skærven, K. Vasconcelos, V. M. Mar Drugs Full Original Paper Aqueous extracts and organic solvent extracts of isolated marine cyanobacteria strains were tested for antimicrobial activity against a fungus, Gram-positive and Gram-negative bacteria and for cytotoxic activity against primary rat hepatocytes and HL-60 cells. Antimicrobial activity was based on the agar diffusion assay. Cytotoxic activity was measured by apoptotic cell death scored by cell surface evaluation and nuclear morphology. A high percentage of apoptotic cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Slight apoptotic effects were observed in primary rat hepatocytes when exposed to aqueous cyanobacterial extracts. Nine cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria, Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. No inhibitory effects were found against the fungus Candida albicans and Gram-negative bacteria. Marine Synechocystis and Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Gram-positive bacteria. The different activity in different extracts suggests different compounds with different polarities. Molecular Diversity Preservation International 2008-01-22 /pmc/articles/PMC2474953/ /pubmed/18648669 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland
spellingShingle Full Original Paper
Martins, R. F.
Ramos, M. F.
Herfindal, L.
Sousa, J. A.
Skærven, K.
Vasconcelos, V. M.
Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
title Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
title_full Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
title_fullStr Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
title_full_unstemmed Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
title_short Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus
title_sort antimicrobial and cytotoxic assessment of marine cyanobacteria - synechocystis and synechococcus
topic Full Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2474953/
https://www.ncbi.nlm.nih.gov/pubmed/18648669
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