Nuclear delivery of NFκB-assisted DNA/polymer complexes: plasmid DNA quantitation by confocal laser scanning microscopy and evidence of nuclear polyplexes by FRET imaging

Quantification of a plasmid DNA (pDNA) and investigation of its polymer-associated state in the nucleus are crucial to evaluate the effectiveness of a gene-delivery system. This study was conducted with p3NF-luc-3NF, a pDNA-bearing optimized κB motif to favour NFκB-driven nuclear import. Here, a qua...

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Detalles Bibliográficos
Autores principales: Breuzard, Gilles, Tertil, Magdalena, Gonçalves, Cristine, Cheradame, Hervé, Géguan, Philippe, Pichon, Chantal, Midoux, Patrick
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475639/
https://www.ncbi.nlm.nih.gov/pubmed/18515353
http://dx.doi.org/10.1093/nar/gkn287

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475639/
https://www.ncbi.nlm.nih.gov/pubmed/18515353
http://dx.doi.org/10.1093/nar/gkn287

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