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Sliding and jumping of single EcoRV restriction enzymes on non-cognate DNA
The restriction endonuclease EcoRV can rapidly locate a short recognition site within long non-cognate DNA using ‘facilitated diffusion’. This process has long been attributed to a sliding mechanism, in which the enzyme first binds to the DNA via nonspecific interaction and then moves along the DNA...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475641/ https://www.ncbi.nlm.nih.gov/pubmed/18544605 http://dx.doi.org/10.1093/nar/gkn376 |