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Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver and Kidneys of Chronically Diabetic Rats
In the present study, the role of endothelin-1 (ET-1) on alterations of hepatic and renal metallothionein (MT) and trace metals (Zn, Cu, and Fe) were investigated in streptozotocin (STZ)- induced diabetic rats. Diabetic rats, age- and sex-matched controls, as well as control and diabetic animals on...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2002
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478585/ https://www.ncbi.nlm.nih.gov/pubmed/12458661 http://dx.doi.org/10.1080/15604280214281 |
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author | Cai, Lu Chen, Shali Evans, Terry Cherian, M. George Chakrabarti, Subrata |
author_facet | Cai, Lu Chen, Shali Evans, Terry Cherian, M. George Chakrabarti, Subrata |
author_sort | Cai, Lu |
collection | PubMed |
description | In the present study, the role of endothelin-1 (ET-1) on alterations of hepatic and renal metallothionein (MT) and trace metals (Zn, Cu, and Fe) were investigated in streptozotocin (STZ)- induced diabetic rats. Diabetic rats, age- and sex-matched controls, as well as control and diabetic animals on a dual ET(A)/ET(B) receptor blocker, bosentan, were investigated after 6 months of follow-up. MT was measured by cadmium-heme assay. Metals were measured by atomic absorption spectrometer. ET-1 mRNA was analyzed by reverse transcriptase–polymerase chain reaction (RT-PCR) technique. Hepatic and renal ET-1 mRNA was increased in diabetic rats as compared to control rats, along with an increase in both hepatic and renal MT proteins. The increased hepatic MT protein level was associated with decreases in hepatic Cu and Fe, whereas increased renal MT was associated with increases in renal Cu and Fe accumulation. Zn levels were unaltered in both organs in diabetic rats. Bosentan treatment partially prevented the increase in MT levels in both liver and kidney, along with reduced serum creatinine and increased urinary creatinine levels. Further bosentan treatment corrected the increased Cu and Fe levels in the kidney in diabetic rats, but reduced hepatic Cu and Fe levels. No significant effects of bosentan treatment on nondiabetic rats were observed. The data suggest that the possible effects of ET antagonism in diabetes may be mediated via changes in MT and trace metals. |
format | Text |
id | pubmed-2478585 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-24785852008-08-18 Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver and Kidneys of Chronically Diabetic Rats Cai, Lu Chen, Shali Evans, Terry Cherian, M. George Chakrabarti, Subrata Int J Exp Diabetes Res Research Article In the present study, the role of endothelin-1 (ET-1) on alterations of hepatic and renal metallothionein (MT) and trace metals (Zn, Cu, and Fe) were investigated in streptozotocin (STZ)- induced diabetic rats. Diabetic rats, age- and sex-matched controls, as well as control and diabetic animals on a dual ET(A)/ET(B) receptor blocker, bosentan, were investigated after 6 months of follow-up. MT was measured by cadmium-heme assay. Metals were measured by atomic absorption spectrometer. ET-1 mRNA was analyzed by reverse transcriptase–polymerase chain reaction (RT-PCR) technique. Hepatic and renal ET-1 mRNA was increased in diabetic rats as compared to control rats, along with an increase in both hepatic and renal MT proteins. The increased hepatic MT protein level was associated with decreases in hepatic Cu and Fe, whereas increased renal MT was associated with increases in renal Cu and Fe accumulation. Zn levels were unaltered in both organs in diabetic rats. Bosentan treatment partially prevented the increase in MT levels in both liver and kidney, along with reduced serum creatinine and increased urinary creatinine levels. Further bosentan treatment corrected the increased Cu and Fe levels in the kidney in diabetic rats, but reduced hepatic Cu and Fe levels. No significant effects of bosentan treatment on nondiabetic rats were observed. The data suggest that the possible effects of ET antagonism in diabetes may be mediated via changes in MT and trace metals. Hindawi Publishing Corporation 2002 /pmc/articles/PMC2478585/ /pubmed/12458661 http://dx.doi.org/10.1080/15604280214281 Text en Copyright © 2002 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cai, Lu Chen, Shali Evans, Terry Cherian, M. George Chakrabarti, Subrata Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver and Kidneys of Chronically Diabetic Rats |
title | Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver
and Kidneys of Chronically Diabetic Rats |
title_full | Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver
and Kidneys of Chronically Diabetic Rats |
title_fullStr | Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver
and Kidneys of Chronically Diabetic Rats |
title_full_unstemmed | Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver
and Kidneys of Chronically Diabetic Rats |
title_short | Endothelin-1–Mediated Alteration of Metallothionein and Trace Metals in the Liver
and Kidneys of Chronically Diabetic Rats |
title_sort | endothelin-1–mediated alteration of metallothionein and trace metals in the liver
and kidneys of chronically diabetic rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478585/ https://www.ncbi.nlm.nih.gov/pubmed/12458661 http://dx.doi.org/10.1080/15604280214281 |
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