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Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection

BACKGROUND: Virus infection induces the activation and suppression of global gene expression in the host. Profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. In this study, the...

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Autores principales: Babu, Mohan, Griffiths, Jonathan S, Huang, Tyng-Shyan, Wang, Aiming
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478689/
https://www.ncbi.nlm.nih.gov/pubmed/18613973
http://dx.doi.org/10.1186/1471-2164-9-325
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author Babu, Mohan
Griffiths, Jonathan S
Huang, Tyng-Shyan
Wang, Aiming
author_facet Babu, Mohan
Griffiths, Jonathan S
Huang, Tyng-Shyan
Wang, Aiming
author_sort Babu, Mohan
collection PubMed
description BACKGROUND: Virus infection induces the activation and suppression of global gene expression in the host. Profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. In this study, the Arabidopsis Affymetrix ATH1 array was used to assess global gene expression changes in Arabidopsis thaliana plants infected with Plum pox virus (PPV). To identify early genes in response to PPV infection, an Arabidopsis synchronized single-cell transformation system was developed. Arabidopsis protoplasts were transfected with a PPV infectious clone and global gene expression changes in the transfected protoplasts were profiled. RESULTS: Microarray analysis of PPV-infected Arabidopsis leaf tissues identified 2013 and 1457 genes that were significantly (Q ≤ 0.05) up- (≥ 2.5 fold) and downregulated (≤ -2.5 fold), respectively. Genes associated with soluble sugar, starch and amino acid, intracellular membrane/membrane-bound organelles, chloroplast, and protein fate were upregulated, while genes related to development/storage proteins, protein synthesis and translation, and cell wall-associated components were downregulated. These gene expression changes were associated with PPV infection and symptom development. Further transcriptional profiling of protoplasts transfected with a PPV infectious clone revealed the upregulation of defence and cellular signalling genes as early as 6 hours post transfection. A cross sequence comparison analysis of genes differentially regulated by PPV-infected Arabidopsis leaves against uniEST sequences derived from PPV-infected leaves of Prunus persica, a natural host of PPV, identified orthologs related to defence, metabolism and protein synthesis. The cross comparison of genes differentially regulated by PPV infection and by the infections of other positive sense RNA viruses revealed a common set of 416 genes. These identified genes, particularly the early responsive genes, may be critical in virus infection. CONCLUSION: Gene expression changes in PPV-infected Arabidopsis are the molecular basis of stress and defence-like responses, PPV pathogenesis and symptom development. The differentially regulated genes, particularly the early responsive genes, and a common set of genes regulated by infections of PPV and other positive sense RNA viruses identified in this study are candidates suitable for further functional characterization to shed lights on molecular virus-host interactions.
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spelling pubmed-24786892008-07-22 Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection Babu, Mohan Griffiths, Jonathan S Huang, Tyng-Shyan Wang, Aiming BMC Genomics Research Article BACKGROUND: Virus infection induces the activation and suppression of global gene expression in the host. Profiling gene expression changes in the host may provide insights into the molecular mechanisms that underlie host physiological and phenotypic responses to virus infection. In this study, the Arabidopsis Affymetrix ATH1 array was used to assess global gene expression changes in Arabidopsis thaliana plants infected with Plum pox virus (PPV). To identify early genes in response to PPV infection, an Arabidopsis synchronized single-cell transformation system was developed. Arabidopsis protoplasts were transfected with a PPV infectious clone and global gene expression changes in the transfected protoplasts were profiled. RESULTS: Microarray analysis of PPV-infected Arabidopsis leaf tissues identified 2013 and 1457 genes that were significantly (Q ≤ 0.05) up- (≥ 2.5 fold) and downregulated (≤ -2.5 fold), respectively. Genes associated with soluble sugar, starch and amino acid, intracellular membrane/membrane-bound organelles, chloroplast, and protein fate were upregulated, while genes related to development/storage proteins, protein synthesis and translation, and cell wall-associated components were downregulated. These gene expression changes were associated with PPV infection and symptom development. Further transcriptional profiling of protoplasts transfected with a PPV infectious clone revealed the upregulation of defence and cellular signalling genes as early as 6 hours post transfection. A cross sequence comparison analysis of genes differentially regulated by PPV-infected Arabidopsis leaves against uniEST sequences derived from PPV-infected leaves of Prunus persica, a natural host of PPV, identified orthologs related to defence, metabolism and protein synthesis. The cross comparison of genes differentially regulated by PPV infection and by the infections of other positive sense RNA viruses revealed a common set of 416 genes. These identified genes, particularly the early responsive genes, may be critical in virus infection. CONCLUSION: Gene expression changes in PPV-infected Arabidopsis are the molecular basis of stress and defence-like responses, PPV pathogenesis and symptom development. The differentially regulated genes, particularly the early responsive genes, and a common set of genes regulated by infections of PPV and other positive sense RNA viruses identified in this study are candidates suitable for further functional characterization to shed lights on molecular virus-host interactions. BioMed Central 2008-07-09 /pmc/articles/PMC2478689/ /pubmed/18613973 http://dx.doi.org/10.1186/1471-2164-9-325 Text en Copyright © 2008 Babu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Babu, Mohan
Griffiths, Jonathan S
Huang, Tyng-Shyan
Wang, Aiming
Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection
title Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection
title_full Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection
title_fullStr Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection
title_full_unstemmed Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection
title_short Altered gene expression changes in Arabidopsis leaf tissues and protoplasts in response to Plum pox virus infection
title_sort altered gene expression changes in arabidopsis leaf tissues and protoplasts in response to plum pox virus infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478689/
https://www.ncbi.nlm.nih.gov/pubmed/18613973
http://dx.doi.org/10.1186/1471-2164-9-325
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