Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site

A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn–Met–Thr at residues 34–36, was experimentally confirmed. The replacement of leucine at pos...

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Detalles Bibliográficos
Autores principales: Gautschi, Matthias, Beintema, Jaap J.
Formato: Texto
Lenguaje:English
Publicado: Springer US 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480611/
https://www.ncbi.nlm.nih.gov/pubmed/18437553
http://dx.doi.org/10.1007/s10528-008-9162-x
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author Gautschi, Matthias
Beintema, Jaap J.
author_facet Gautschi, Matthias
Beintema, Jaap J.
author_sort Gautschi, Matthias
collection PubMed
description A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn–Met–Thr at residues 34–36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.
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spelling pubmed-24806112008-07-22 Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site Gautschi, Matthias Beintema, Jaap J. Biochem Genet Article A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn–Met–Thr at residues 34–36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells. Springer US 2008-04-24 2008 /pmc/articles/PMC2480611/ /pubmed/18437553 http://dx.doi.org/10.1007/s10528-008-9162-x Text en © The Author(s) 2008 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Gautschi, Matthias
Beintema, Jaap J.
Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site
title Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site
title_full Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site
title_fullStr Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site
title_full_unstemmed Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site
title_short Selection Against Glycosylation in Ruminant Pancreatic Ribonucleases by Replacements in the Ancestral Carbohydrate Attachment Site
title_sort selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2480611/
https://www.ncbi.nlm.nih.gov/pubmed/18437553
http://dx.doi.org/10.1007/s10528-008-9162-x
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