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Chætognath transcriptome reveals ancestral and unique features among bilaterians

BACKGROUND: The chætognaths (arrow worms) have puzzled zoologists for years because of their astonishing morphological and developmental characteristics. Despite their deuterostome-like development, phylogenomic studies recently positioned the chætognath phylum in protostomes, most likely in an earl...

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Autores principales: Marlétaz, Ferdinand, Gilles, André, Caubit, Xavier, Perez, Yvan, Dossat, Carole, Samain, Sylvie, Gyapay, Gabor, Wincker, Patrick, Le Parco, Yannick
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2481426/
https://www.ncbi.nlm.nih.gov/pubmed/18533022
http://dx.doi.org/10.1186/gb-2008-9-6-r94
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author Marlétaz, Ferdinand
Gilles, André
Caubit, Xavier
Perez, Yvan
Dossat, Carole
Samain, Sylvie
Gyapay, Gabor
Wincker, Patrick
Le Parco, Yannick
author_facet Marlétaz, Ferdinand
Gilles, André
Caubit, Xavier
Perez, Yvan
Dossat, Carole
Samain, Sylvie
Gyapay, Gabor
Wincker, Patrick
Le Parco, Yannick
author_sort Marlétaz, Ferdinand
collection PubMed
description BACKGROUND: The chætognaths (arrow worms) have puzzled zoologists for years because of their astonishing morphological and developmental characteristics. Despite their deuterostome-like development, phylogenomic studies recently positioned the chætognath phylum in protostomes, most likely in an early branching. This key phylogenetic position and the peculiar characteristics of chætognaths prompted further investigation of their genomic features. RESULTS: Transcriptomic and genomic data were collected from the chætognath Spadella cephaloptera through the sequencing of expressed sequence tags and genomic bacterial artificial chromosome clones. Transcript comparisons at various taxonomic scales emphasized the conservation of a core gene set and phylogenomic analysis confirmed the basal position of chætognaths among protostomes. A detailed survey of transcript diversity and individual genotyping revealed a past genome duplication event in the chætognath lineage, which was, surprisingly, followed by a high retention rate of duplicated genes. Moreover, striking genetic heterogeneity was detected within the sampled population at the nuclear and mitochondrial levels but cannot be explained by cryptic speciation. Finally, we found evidence for trans-splicing maturation of transcripts through splice-leader addition in the chætognath phylum and we further report that this processing is associated with operonic transcription. CONCLUSION: These findings reveal both shared ancestral and unique derived characteristics of the chætognath genome, which suggests that this genome is likely the product of a very original evolutionary history. These features promote chætognaths as a pivotal model for comparative genomics, which could provide new clues for the investigation of the evolution of animal genomes.
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spelling pubmed-24814262008-07-24 Chætognath transcriptome reveals ancestral and unique features among bilaterians Marlétaz, Ferdinand Gilles, André Caubit, Xavier Perez, Yvan Dossat, Carole Samain, Sylvie Gyapay, Gabor Wincker, Patrick Le Parco, Yannick Genome Biol Research BACKGROUND: The chætognaths (arrow worms) have puzzled zoologists for years because of their astonishing morphological and developmental characteristics. Despite their deuterostome-like development, phylogenomic studies recently positioned the chætognath phylum in protostomes, most likely in an early branching. This key phylogenetic position and the peculiar characteristics of chætognaths prompted further investigation of their genomic features. RESULTS: Transcriptomic and genomic data were collected from the chætognath Spadella cephaloptera through the sequencing of expressed sequence tags and genomic bacterial artificial chromosome clones. Transcript comparisons at various taxonomic scales emphasized the conservation of a core gene set and phylogenomic analysis confirmed the basal position of chætognaths among protostomes. A detailed survey of transcript diversity and individual genotyping revealed a past genome duplication event in the chætognath lineage, which was, surprisingly, followed by a high retention rate of duplicated genes. Moreover, striking genetic heterogeneity was detected within the sampled population at the nuclear and mitochondrial levels but cannot be explained by cryptic speciation. Finally, we found evidence for trans-splicing maturation of transcripts through splice-leader addition in the chætognath phylum and we further report that this processing is associated with operonic transcription. CONCLUSION: These findings reveal both shared ancestral and unique derived characteristics of the chætognath genome, which suggests that this genome is likely the product of a very original evolutionary history. These features promote chætognaths as a pivotal model for comparative genomics, which could provide new clues for the investigation of the evolution of animal genomes. BioMed Central 2008 2008-06-04 /pmc/articles/PMC2481426/ /pubmed/18533022 http://dx.doi.org/10.1186/gb-2008-9-6-r94 Text en Copyright © 2008 Marlétaz et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Marlétaz, Ferdinand
Gilles, André
Caubit, Xavier
Perez, Yvan
Dossat, Carole
Samain, Sylvie
Gyapay, Gabor
Wincker, Patrick
Le Parco, Yannick
Chætognath transcriptome reveals ancestral and unique features among bilaterians
title Chætognath transcriptome reveals ancestral and unique features among bilaterians
title_full Chætognath transcriptome reveals ancestral and unique features among bilaterians
title_fullStr Chætognath transcriptome reveals ancestral and unique features among bilaterians
title_full_unstemmed Chætognath transcriptome reveals ancestral and unique features among bilaterians
title_short Chætognath transcriptome reveals ancestral and unique features among bilaterians
title_sort chætognath transcriptome reveals ancestral and unique features among bilaterians
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2481426/
https://www.ncbi.nlm.nih.gov/pubmed/18533022
http://dx.doi.org/10.1186/gb-2008-9-6-r94
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