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Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A

To assemble mitotic spindles, cells nucleate microtubules from a variety of sources including chromosomes and centrosomes. We know little about how the regulation of microtubule nucleation contributes to spindle bipolarity and spindle size. The Aurora A kinase activator TPX2 is required for microtub...

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Detalles Bibliográficos
Autores principales: Bird, Alexander W., Hyman, Anthony A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483532/
https://www.ncbi.nlm.nih.gov/pubmed/18663142
http://dx.doi.org/10.1083/jcb.200802005
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author Bird, Alexander W.
Hyman, Anthony A.
author_facet Bird, Alexander W.
Hyman, Anthony A.
author_sort Bird, Alexander W.
collection PubMed
description To assemble mitotic spindles, cells nucleate microtubules from a variety of sources including chromosomes and centrosomes. We know little about how the regulation of microtubule nucleation contributes to spindle bipolarity and spindle size. The Aurora A kinase activator TPX2 is required for microtubule nucleation from chromosomes as well as for spindle bipolarity. We use bacterial artificial chromosome–based recombineering to introduce point mutants that block the interaction between TPX2 and Aurora A into human cells. TPX2 mutants have very short spindles but, surprisingly, are still bipolar and segregate chromosomes. Examination of microtubule nucleation during spindle assembly shows that microtubules fail to nucleate from chromosomes. Thus, chromosome nucleation is not essential for bipolarity during human cell mitosis when centrosomes are present. Rather, chromosome nucleation is involved in spindle pole separation and setting spindle length. A second Aurora A–independent function of TPX2 is required to bipolarize spindles.
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spelling pubmed-24835322009-01-28 Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A Bird, Alexander W. Hyman, Anthony A. J Cell Biol Research Articles To assemble mitotic spindles, cells nucleate microtubules from a variety of sources including chromosomes and centrosomes. We know little about how the regulation of microtubule nucleation contributes to spindle bipolarity and spindle size. The Aurora A kinase activator TPX2 is required for microtubule nucleation from chromosomes as well as for spindle bipolarity. We use bacterial artificial chromosome–based recombineering to introduce point mutants that block the interaction between TPX2 and Aurora A into human cells. TPX2 mutants have very short spindles but, surprisingly, are still bipolar and segregate chromosomes. Examination of microtubule nucleation during spindle assembly shows that microtubules fail to nucleate from chromosomes. Thus, chromosome nucleation is not essential for bipolarity during human cell mitosis when centrosomes are present. Rather, chromosome nucleation is involved in spindle pole separation and setting spindle length. A second Aurora A–independent function of TPX2 is required to bipolarize spindles. The Rockefeller University Press 2008-07-28 /pmc/articles/PMC2483532/ /pubmed/18663142 http://dx.doi.org/10.1083/jcb.200802005 Text en © 2008 Bird and Hyman This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Bird, Alexander W.
Hyman, Anthony A.
Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
title Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
title_full Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
title_fullStr Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
title_full_unstemmed Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
title_short Building a spindle of the correct length in human cells requires the interaction between TPX2 and Aurora A
title_sort building a spindle of the correct length in human cells requires the interaction between tpx2 and aurora a
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483532/
https://www.ncbi.nlm.nih.gov/pubmed/18663142
http://dx.doi.org/10.1083/jcb.200802005
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