Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore

BACKGROUND: In Singapore, an outbreak of fungal keratitis caused by members of the Fusarium solani species complex (FSSC) was identified in March 2005 to May 2006 involving 66 patients. Epidemiological investigations have indicated that improper contact lens wear and the use of specific contact lens...

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Autores principales: Jureen, Roland, Koh, Tse H, Wang, Grace, Chai, Louis YA, Tan, Ai L, Chai, Tracy, Wong, Yong W, Wang, Yue, Tambyah, Paul A, Beuerman, Roger, Tan, Donald
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483985/
https://www.ncbi.nlm.nih.gov/pubmed/18627616
http://dx.doi.org/10.1186/1471-2334-8-92
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author Jureen, Roland
Koh, Tse H
Wang, Grace
Chai, Louis YA
Tan, Ai L
Chai, Tracy
Wong, Yong W
Wang, Yue
Tambyah, Paul A
Beuerman, Roger
Tan, Donald
author_facet Jureen, Roland
Koh, Tse H
Wang, Grace
Chai, Louis YA
Tan, Ai L
Chai, Tracy
Wong, Yong W
Wang, Yue
Tambyah, Paul A
Beuerman, Roger
Tan, Donald
author_sort Jureen, Roland
collection PubMed
description BACKGROUND: In Singapore, an outbreak of fungal keratitis caused by members of the Fusarium solani species complex (FSSC) was identified in March 2005 to May 2006 involving 66 patients. Epidemiological investigations have indicated that improper contact lens wear and the use of specific contact lens solutions were risk factors. METHODS: We assessed the genetic diversity of the isolates using AFLP, Rep-PCR, and ERIC-PCR and compared the usefulness of these typing schemes to characterize the isolates. RESULTS: AFLP was the most discriminative typing scheme and appears to group FSSC from eye infections and from other infections differently. CONCLUSION: There was a high genomic heterogeneity among the isolates confirming that this was not a point source outbreak.
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spelling pubmed-24839852008-07-26 Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore Jureen, Roland Koh, Tse H Wang, Grace Chai, Louis YA Tan, Ai L Chai, Tracy Wong, Yong W Wang, Yue Tambyah, Paul A Beuerman, Roger Tan, Donald BMC Infect Dis Research Article BACKGROUND: In Singapore, an outbreak of fungal keratitis caused by members of the Fusarium solani species complex (FSSC) was identified in March 2005 to May 2006 involving 66 patients. Epidemiological investigations have indicated that improper contact lens wear and the use of specific contact lens solutions were risk factors. METHODS: We assessed the genetic diversity of the isolates using AFLP, Rep-PCR, and ERIC-PCR and compared the usefulness of these typing schemes to characterize the isolates. RESULTS: AFLP was the most discriminative typing scheme and appears to group FSSC from eye infections and from other infections differently. CONCLUSION: There was a high genomic heterogeneity among the isolates confirming that this was not a point source outbreak. BioMed Central 2008-07-15 /pmc/articles/PMC2483985/ /pubmed/18627616 http://dx.doi.org/10.1186/1471-2334-8-92 Text en Copyright © 2008 Jureen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jureen, Roland
Koh, Tse H
Wang, Grace
Chai, Louis YA
Tan, Ai L
Chai, Tracy
Wong, Yong W
Wang, Yue
Tambyah, Paul A
Beuerman, Roger
Tan, Donald
Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore
title Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore
title_full Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore
title_fullStr Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore
title_full_unstemmed Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore
title_short Use of multiple methods for genotyping Fusarium during an outbreak of contact lens associated fungal keratitis in Singapore
title_sort use of multiple methods for genotyping fusarium during an outbreak of contact lens associated fungal keratitis in singapore
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2483985/
https://www.ncbi.nlm.nih.gov/pubmed/18627616
http://dx.doi.org/10.1186/1471-2334-8-92
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