Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes

We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake. We then compared two different strategies for DC matura...

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Detalles Bibliográficos
Autores principales: Obermaier, Bianca, Dauer, Marc, Herten, Jan, Schad, Katharina, Endres, Stefan, Eigler, Andreas
Formato: Texto
Lenguaje:English
Publicado: Biological Procedures Online 2003
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC248478/
https://www.ncbi.nlm.nih.gov/pubmed/14615816
http://dx.doi.org/10.1251/bpo62
Descripción
Sumario:We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake. We then compared two different strategies for DC maturation: proinflammatory mediators were either added together with GM-CSF and IL-4 from the beginning of cell culture or added after 24 hours of differentiation with GM-CSF and IL-4. After 48 hours of total culture period, expression of activation markers was more pronounced in cells generated by the 2-step differentiation and activation method. Our new protocol for 2-day DC differentiation reduces labor, cost and time and also reliably renders high numbers of mature and viable DCs.

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