Cargando…

Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules

Microtubules are a major structural component of the cytoskeleton and participate in cell division, intracellular transport, and cell morphogenesis. In the present study, 795 cotton tubulin expressed sequence tags were analysed and 19 β-tubulin genes (TUB) cloned from a cotton cDNA library. Among th...

Descripción completa

Detalles Bibliográficos
Autores principales: He, Xian-Chen, Qin, Yong-Mei, Xu, Yu, Hu, Chun-Yang, Zhu, Yu-Xian
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2486464/
https://www.ncbi.nlm.nih.gov/pubmed/18596112
http://dx.doi.org/10.1093/jxb/ern127
_version_ 1782158104381620224
author He, Xian-Chen
Qin, Yong-Mei
Xu, Yu
Hu, Chun-Yang
Zhu, Yu-Xian
author_facet He, Xian-Chen
Qin, Yong-Mei
Xu, Yu
Hu, Chun-Yang
Zhu, Yu-Xian
author_sort He, Xian-Chen
collection PubMed
description Microtubules are a major structural component of the cytoskeleton and participate in cell division, intracellular transport, and cell morphogenesis. In the present study, 795 cotton tubulin expressed sequence tags were analysed and 19 β-tubulin genes (TUB) cloned from a cotton cDNA library. Among the group, 12 cotton TUBs (GhTUBs) are reported for the first time here. Transcription profiling revealed that nine GhTUBs were highly expressed in elongating fibre cells as compared with fuzzless-lintless mutant ovules. Treating cultured wild-type cotton ovules with exogenous phytohormones showed that individual genes can be induced by different agents. Gibberellin induced expression of GhTUB1 and GhTUB3, ethylene induced expression of GhTUB5, GhTUB9, and GhTUB12, brassinosteroids induced expression of GhTUB1, GhTUB3, GhTUB9, and GhTUB12, and lignoceric acid induced expression of GhTUB1, GhTUB3, and GhTUB12. When GhTUBs were transformed into the Saccharomyces cerevisiae inviable mutant, tub2, which is deficient in β-tubulin, one ovule-specific and eight of nine fibre-preferential GhTUBs rescued this lethality. This study suggests that the proteins encoded by cotton GhTUBs are involved during cotton fibre development.
format Text
id pubmed-2486464
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-24864642009-02-25 Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules He, Xian-Chen Qin, Yong-Mei Xu, Yu Hu, Chun-Yang Zhu, Yu-Xian J Exp Bot Research Papers Microtubules are a major structural component of the cytoskeleton and participate in cell division, intracellular transport, and cell morphogenesis. In the present study, 795 cotton tubulin expressed sequence tags were analysed and 19 β-tubulin genes (TUB) cloned from a cotton cDNA library. Among the group, 12 cotton TUBs (GhTUBs) are reported for the first time here. Transcription profiling revealed that nine GhTUBs were highly expressed in elongating fibre cells as compared with fuzzless-lintless mutant ovules. Treating cultured wild-type cotton ovules with exogenous phytohormones showed that individual genes can be induced by different agents. Gibberellin induced expression of GhTUB1 and GhTUB3, ethylene induced expression of GhTUB5, GhTUB9, and GhTUB12, brassinosteroids induced expression of GhTUB1, GhTUB3, GhTUB9, and GhTUB12, and lignoceric acid induced expression of GhTUB1, GhTUB3, and GhTUB12. When GhTUBs were transformed into the Saccharomyces cerevisiae inviable mutant, tub2, which is deficient in β-tubulin, one ovule-specific and eight of nine fibre-preferential GhTUBs rescued this lethality. This study suggests that the proteins encoded by cotton GhTUBs are involved during cotton fibre development. Oxford University Press 2008-07 /pmc/articles/PMC2486464/ /pubmed/18596112 http://dx.doi.org/10.1093/jxb/ern127 Text en © 2008 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
He, Xian-Chen
Qin, Yong-Mei
Xu, Yu
Hu, Chun-Yang
Zhu, Yu-Xian
Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules
title Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules
title_full Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules
title_fullStr Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules
title_full_unstemmed Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules
title_short Molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cDNAs from developing cotton ovules
title_sort molecular cloning, expression profiling, and yeast complementation of 19 β-tubulin cdnas from developing cotton ovules
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2486464/
https://www.ncbi.nlm.nih.gov/pubmed/18596112
http://dx.doi.org/10.1093/jxb/ern127
work_keys_str_mv AT hexianchen molecularcloningexpressionprofilingandyeastcomplementationof19btubulincdnasfromdevelopingcottonovules
AT qinyongmei molecularcloningexpressionprofilingandyeastcomplementationof19btubulincdnasfromdevelopingcottonovules
AT xuyu molecularcloningexpressionprofilingandyeastcomplementationof19btubulincdnasfromdevelopingcottonovules
AT huchunyang molecularcloningexpressionprofilingandyeastcomplementationof19btubulincdnasfromdevelopingcottonovules
AT zhuyuxian molecularcloningexpressionprofilingandyeastcomplementationof19btubulincdnasfromdevelopingcottonovules