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Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India

BACKGROUND: Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% of patients. Why a subset of malaria patients develops CM complications, including neurological sequelae or death, is still not well understo...

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Autores principales: Lucchi, Naomi W, Tongren, Jon Eric, Jain, Vidhan, Nagpal, Avinash C, Kauth, Christian W, Woehlbier, Ute, Bujard, Hermann, Dash, Aditya P, Singh, Neeru, Stiles, Jonathan K, Udhayakumar, Venkatachalam
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2491629/
https://www.ncbi.nlm.nih.gov/pubmed/18601721
http://dx.doi.org/10.1186/1475-2875-7-121
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author Lucchi, Naomi W
Tongren, Jon Eric
Jain, Vidhan
Nagpal, Avinash C
Kauth, Christian W
Woehlbier, Ute
Bujard, Hermann
Dash, Aditya P
Singh, Neeru
Stiles, Jonathan K
Udhayakumar, Venkatachalam
author_facet Lucchi, Naomi W
Tongren, Jon Eric
Jain, Vidhan
Nagpal, Avinash C
Kauth, Christian W
Woehlbier, Ute
Bujard, Hermann
Dash, Aditya P
Singh, Neeru
Stiles, Jonathan K
Udhayakumar, Venkatachalam
author_sort Lucchi, Naomi W
collection PubMed
description BACKGROUND: Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% of patients. Why a subset of malaria patients develops CM complications, including neurological sequelae or death, is still not well understood. It is believed that host immune factors may modulate CM outcomes and there is substantial evidence that cellular immune factors, such as cytokines, play an important role in this process. In this study, the potential relationship between the antibody responses to the merozoite surface protein (MSP)-1 complex (which consists of four fragments namely: MSP-1(83), MSP-1(30), MSP-1(38 )and MSP-1(42)), MSP-6(36 )and MSP-7(22 )and CM was investigated. METHODS: Peripheral blood antibody responses to recombinant antigens of the two major allelic forms of MSP-1 complex, MSP-6(36 )and MSP-7(22 )were compared between healthy subjects, mild malaria patients (MM) and CM patients residing in a malaria endemic region of central India. Total IgG and IgG subclass antibody responses were determined using ELISA method. RESULTS: The prevalence and levels of IgG and its subclasses in the plasma varied for each antigen. In general, the prevalence of total IgG, IgG1 and IgG3 was higher in the MM patients and lower in CM patients compared to healthy controls. Significantly lower levels of total IgG antibodies to the MSP-1(f38), IgG1 levels to MSP-1(d83), MSP-1(19 )and MSP-6(36 )and IgG3 levels to MSP-1(f42 )and MSP-7(22 )were observed in CM patients as compared to MM patients. CONCLUSION: These results suggest that there may be some dysregulation in the generation of antibody responses to some MSP antigens in CM patients and it is worth investigating further whether perturbations of antibody responses in CM patients contribute to pathogenesis.
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spelling pubmed-24916292008-07-31 Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India Lucchi, Naomi W Tongren, Jon Eric Jain, Vidhan Nagpal, Avinash C Kauth, Christian W Woehlbier, Ute Bujard, Hermann Dash, Aditya P Singh, Neeru Stiles, Jonathan K Udhayakumar, Venkatachalam Malar J Research BACKGROUND: Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% of patients. Why a subset of malaria patients develops CM complications, including neurological sequelae or death, is still not well understood. It is believed that host immune factors may modulate CM outcomes and there is substantial evidence that cellular immune factors, such as cytokines, play an important role in this process. In this study, the potential relationship between the antibody responses to the merozoite surface protein (MSP)-1 complex (which consists of four fragments namely: MSP-1(83), MSP-1(30), MSP-1(38 )and MSP-1(42)), MSP-6(36 )and MSP-7(22 )and CM was investigated. METHODS: Peripheral blood antibody responses to recombinant antigens of the two major allelic forms of MSP-1 complex, MSP-6(36 )and MSP-7(22 )were compared between healthy subjects, mild malaria patients (MM) and CM patients residing in a malaria endemic region of central India. Total IgG and IgG subclass antibody responses were determined using ELISA method. RESULTS: The prevalence and levels of IgG and its subclasses in the plasma varied for each antigen. In general, the prevalence of total IgG, IgG1 and IgG3 was higher in the MM patients and lower in CM patients compared to healthy controls. Significantly lower levels of total IgG antibodies to the MSP-1(f38), IgG1 levels to MSP-1(d83), MSP-1(19 )and MSP-6(36 )and IgG3 levels to MSP-1(f42 )and MSP-7(22 )were observed in CM patients as compared to MM patients. CONCLUSION: These results suggest that there may be some dysregulation in the generation of antibody responses to some MSP antigens in CM patients and it is worth investigating further whether perturbations of antibody responses in CM patients contribute to pathogenesis. BioMed Central 2008-07-04 /pmc/articles/PMC2491629/ /pubmed/18601721 http://dx.doi.org/10.1186/1475-2875-7-121 Text en Copyright © 2008 Lucchi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lucchi, Naomi W
Tongren, Jon Eric
Jain, Vidhan
Nagpal, Avinash C
Kauth, Christian W
Woehlbier, Ute
Bujard, Hermann
Dash, Aditya P
Singh, Neeru
Stiles, Jonathan K
Udhayakumar, Venkatachalam
Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
title Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
title_full Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
title_fullStr Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
title_full_unstemmed Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
title_short Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India
title_sort antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in india
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2491629/
https://www.ncbi.nlm.nih.gov/pubmed/18601721
http://dx.doi.org/10.1186/1475-2875-7-121
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