Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro

In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To i...

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Autores principales: Yao, Hailei, Xie, Xiaoyan, Li, Yanhua, Wang, Dongmei, Han, Shu, Shi, Shuangshuang, Nan, Xue, Bai, Cixian, Wang, Yunfang, Pei, Xuetao
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2496955/
https://www.ncbi.nlm.nih.gov/pubmed/18695740
http://dx.doi.org/10.1155/2008/531317
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author Yao, Hailei
Xie, Xiaoyan
Li, Yanhua
Wang, Dongmei
Han, Shu
Shi, Shuangshuang
Nan, Xue
Bai, Cixian
Wang, Yunfang
Pei, Xuetao
author_facet Yao, Hailei
Xie, Xiaoyan
Li, Yanhua
Wang, Dongmei
Han, Shu
Shi, Shuangshuang
Nan, Xue
Bai, Cixian
Wang, Yunfang
Pei, Xuetao
author_sort Yao, Hailei
collection PubMed
description In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation.
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spelling pubmed-24969552008-08-11 Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro Yao, Hailei Xie, Xiaoyan Li, Yanhua Wang, Dongmei Han, Shu Shi, Shuangshuang Nan, Xue Bai, Cixian Wang, Yunfang Pei, Xuetao Clin Dev Immunol Research Article In vitro maintenance of stem cells is crucial for many clinical applications. Stem cell preservation factor FRIL (Flt3 receptor-interacting lectin) is a plant lectin extracted from Dolichos Lablab and has been found preserve hematopoietic stem cells in vitro for a month in our previous studies. To investigate whether FRIL can preserve neural progenitor cells (NPCs), it was supplemented into serum-free suspension culture media. FRIL made NPC grow slowly, induced cell adhesion, and delayed neurospheres formation. However, FRIL did not initiate NPC differentiation according to immunofluorescence and semiquantitive RT-PCR results. In conclusion, FRIL could also preserve neural progenitor cells in vitro by inhibiting both cell proliferation and differentiation. Hindawi Publishing Corporation 2008 2008-08-05 /pmc/articles/PMC2496955/ /pubmed/18695740 http://dx.doi.org/10.1155/2008/531317 Text en Copyright © 2008 Hailei Yao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yao, Hailei
Xie, Xiaoyan
Li, Yanhua
Wang, Dongmei
Han, Shu
Shi, Shuangshuang
Nan, Xue
Bai, Cixian
Wang, Yunfang
Pei, Xuetao
Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro
title Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro
title_full Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro
title_fullStr Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro
title_full_unstemmed Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro
title_short Legume Lectin FRIL Preserves Neural Progenitor Cells in Suspension Culture In Vitro
title_sort legume lectin fril preserves neural progenitor cells in suspension culture in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2496955/
https://www.ncbi.nlm.nih.gov/pubmed/18695740
http://dx.doi.org/10.1155/2008/531317
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