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Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs
The spatial configuration of the chicken α-globin gene domain in erythroid and lymphoid cells was studied by using the Chromosome Conformation Capture (3C) approach. Real-time PCR with TaqMan probes was employed to estimate the frequencies of cross-linking of different restriction fragments within t...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504291/ https://www.ncbi.nlm.nih.gov/pubmed/18621783 http://dx.doi.org/10.1093/nar/gkn429 |
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author | Gavrilov, Alexey A. Razin, Sergey V. |
author_facet | Gavrilov, Alexey A. Razin, Sergey V. |
author_sort | Gavrilov, Alexey A. |
collection | PubMed |
description | The spatial configuration of the chicken α-globin gene domain in erythroid and lymphoid cells was studied by using the Chromosome Conformation Capture (3C) approach. Real-time PCR with TaqMan probes was employed to estimate the frequencies of cross-linking of different restriction fragments within the domain. In differentiated cultured erythroblasts and in 10-day chick embryo erythrocytes expressing ‘adult’ α(A) and α(D) globin genes the following elements of the domain were found to form an ‘active’ chromatin hub: upstream Major Regulatory Element (MRE), −9 kb upstream DNase I hypersensitive site (DHS), −4 kb upstream CpG island, α(D) gene promoter and the downstream enhancer. The α(A) gene promoter was not present in the ‘active’ chromatin hub although the level of α(A) gene transcription exceeded that of the α(D) gene. Formation of the ‘active’ chromatin hub was preceded by the assembly of multiple incomplete hubs containing MRE in combination with either −9 kb DHS or other regulatory elements of the domain. These incomplete chromatin hubs were present in proliferating cultured erythroblasts which did not express globin genes. In lymphoid cells only the interaction between the α(D) promoter and the CpG island was detected. |
format | Text |
id | pubmed-2504291 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25042912008-08-08 Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs Gavrilov, Alexey A. Razin, Sergey V. Nucleic Acids Res Genomics The spatial configuration of the chicken α-globin gene domain in erythroid and lymphoid cells was studied by using the Chromosome Conformation Capture (3C) approach. Real-time PCR with TaqMan probes was employed to estimate the frequencies of cross-linking of different restriction fragments within the domain. In differentiated cultured erythroblasts and in 10-day chick embryo erythrocytes expressing ‘adult’ α(A) and α(D) globin genes the following elements of the domain were found to form an ‘active’ chromatin hub: upstream Major Regulatory Element (MRE), −9 kb upstream DNase I hypersensitive site (DHS), −4 kb upstream CpG island, α(D) gene promoter and the downstream enhancer. The α(A) gene promoter was not present in the ‘active’ chromatin hub although the level of α(A) gene transcription exceeded that of the α(D) gene. Formation of the ‘active’ chromatin hub was preceded by the assembly of multiple incomplete hubs containing MRE in combination with either −9 kb DHS or other regulatory elements of the domain. These incomplete chromatin hubs were present in proliferating cultured erythroblasts which did not express globin genes. In lymphoid cells only the interaction between the α(D) promoter and the CpG island was detected. Oxford University Press 2008-08 2008-07-11 /pmc/articles/PMC2504291/ /pubmed/18621783 http://dx.doi.org/10.1093/nar/gkn429 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genomics Gavrilov, Alexey A. Razin, Sergey V. Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
title | Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
title_full | Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
title_fullStr | Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
title_full_unstemmed | Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
title_short | Spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
title_sort | spatial configuration of the chicken α-globin gene domain: immature and active chromatin hubs |
topic | Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504291/ https://www.ncbi.nlm.nih.gov/pubmed/18621783 http://dx.doi.org/10.1093/nar/gkn429 |
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