Light activation of transcription: photocaging of nucleotides for control over RNA polymerization

We describe the use of ATP caged with [7-(diethylamino)coumarin-4-yl]methyl (DEACM) for light-controlled in vitro transcription reactions. Polymerization is blocked when DEACM is bonded to the gamma phosphate group of the ATP molecule. Controlled light irradiation releases ATP and transcription is i...

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Detalles Bibliográficos
Autores principales: Pinheiro, André Vidal, Baptista, Pedro, Lima, João Carlos
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504310/
https://www.ncbi.nlm.nih.gov/pubmed/18586819
http://dx.doi.org/10.1093/nar/gkn415

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504310/
https://www.ncbi.nlm.nih.gov/pubmed/18586819
http://dx.doi.org/10.1093/nar/gkn415

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